engleski

Mathematical Model for Human High Density Lipoprotein Dynamics

The dynamics of the lipid monolayer was monitored by spin label Tempo incorporated into the lipoprotein via its reduction with ascorbic acid. The reduction of the paramagnetic nitroxide into the nonparamagnetic hydroxylamine form caused the decrease of the ESR signal with time. The experiments clearly demonstrate that the solubilisation of the ambivalent molecule Tempo in the hydrophobic environment of HDL enhanced its reaction kinetics. In order to explain experimental data the model for reduction of Tempo by ascorbic acid is proposed which assumes that the processes taking place in these heterogeneous systems are determined by the overall reaction rate dependent on the local concentrations of the reactants as well as on their transport coefficients for the particular phases. The model was checked by measurements in different concentrations. According to the obtained results we can propose that HDL2 subfraction is more efficient than HDL3 in promoting the reaction what could be related to the difference in atherogenetic protection behavior between these two members of the lipoprotein particles family.

HDL; atherogenesis; spin label

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