engleski

Novel approach in developing Saccharomyces cerevisiae hybrid bioethanol producers by mating of natural isolates having desirable traits

Bioethanol production from lignocellulosic hydrolysates requires a producer strain that tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous intraspecies hybrid diploids of Saccharomyces cerevisiae by crossing two natural S. cerevisiae isolates, a good ethanol producer found in wine and a strain resistant to inhibitors found in lignocellulosic hydrolysates. Compared to parental strains, hybrids grew faster in the absence, as well as in the presence of acetic and levulinic acid and 2-furaldehyde, inhibitors frequently found in lignocellulosic hydrolysates. It was shown that the overexpression of YAP1 gene, involved in a stress response, further increased the survival. Furthermore, hybrids displayed different fermentative potential in a CO2 production test suggesting genetic variability that could be used for further selection of desirable traits. Therefore, our results suggest that the construction of intraspecies hybrids coupled with use of genetic engineering techniques is a promising approach for an improvement or development of new biotechnologically relevant strains of S. cerevisiae.

Sacchromyces cerevisae, hybrids, bioethanol

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