engleski

In vivo Growth Inhibitory and Anti-angiogenic Effects of Gallic Acid on Advanced Erlich Ascites Tumor in Mouse

Macrophage recruitment and cytokine/chemokine production, growth factors and proteolytic enzymes is a common feature of inflammation, angiogenesis and cancer, and an important goal of a new approach to cancer treatment. Gallic acid as natural phytochemical and strong antioxidant shows chemopreventive and therapeutic properties in cancer treatment. We investigated the effect of gallic acid on tumor growth, angiogenesis, macrophage polarization and oxidative stress on the angiogenic model caused by ip inoculation of EAT cells (2.5x106) in Swiss albino mouse. The results show that gallic acid exhibit immunostimulatory, antiinflammatory, antiangiogenic and antitumor effect. The antitumor effect of gallic acid is based on the cytotoxic efficacy of M1 macrophage, direct inhibition of EAT cell growth, inhibition of vascular endothelial growth factors (VEGF) and growth of blood vessels in the peritoneal cavity of EAT- bearing mice. Gallic acid through the inhibition of proangiogenic factors, particularly VEGF, metalloproteinases 2 and 9 (MMP-2 and MMP-9) and cyclooxygenase 2 activity (COX-2), inhibit process of angiogenesis, tumor growth and increase survival of EAT-bearing mice. The antioxidant ability of gallic acid blocks the occurrence of M2 tumor associated macrophages (TAMs) and significantly inhibits tumorigenesis in mouse model. Based on the above, TAMs inhibition may be effective in the cancer treatment and other chronic diseases associated with process of angiogenesis.

angiogenesis ; gallic acid ; macrophage polarization ; proangiogenic factors ; TAMs

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