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The Effect of Sevoflurane on Oxidative Stress and Inflammation Induced by Aluminium Chloride in the Rat Brain

The aim of this study was to investigate possible antioxidant/prooxidative effect of sevoflurane on AlCl3-induced oxidative stress by monitoring:change in oxidoreduction status of the brain by changing the level of glutathione (GSH) and malondialdehyde (MDA) ; activity of catalase (CAT) and superoxide dismutase (SOD) in brain tissue ; evaluation of the neuro- inflammation, by measuring the relative weight of the brain compared to a healthy control group ; analyzing changes in haematological parameters and osmotic fragility of erythrocytes as indicators of the toxicity of AlCl3, sevoflurane and/or these combination. Results show that AlCl3 causes an increased level of oxidative stress ; significantly increases MDA levels and decreased GSH levels in the brain and changes to a lesser extent the activity of antioxidant enzymes compared to sevoflurane. Sevoflurane in combination with AlCl3 exhibits a stronger toxic effect ; MDA level was significantly increased as well as activity of antioxidant enzymes SOD and CAT. Increased neuro-inflammation is visible in group treated with AlCl3, while sevoflurane + AlCl3 cause decaying of brain cells and significant toxic effects on haematological parameters. Based on obtained results we can conclude that significantly increased level of lipid peroxidation in the brain and neuro- inflammation indicates neurotoxicity of AlCl3, while sevoflurane in combination with AlCl3 exhibits a higher toxic effect and a significant degradation of brain cells.

sevoflurane, aluminium chloride, antioxidative system, neuroinflammatory

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