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Biochemical response of Arabidopsis thaliana transgenic plants with a disturbed function of enzyme auxin amidohydrolase after salt stress exposure (CROSBI ID 666303)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Vujčić Bok, Valerija ; Radić Brkanac, Sandra ; Pavlović, Iva ; Linić, Ida ; Salopek-Sondi, Branka Biochemical response of Arabidopsis thaliana transgenic plants with a disturbed function of enzyme auxin amidohydrolase after salt stress exposure // Book of abstracts of the 13th Croatian biological congress / Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja et al. (ur.). Zagreb: Hrvatsko biološko društvo, 2018. str. 215-216

Podaci o odgovornosti

Vujčić Bok, Valerija ; Radić Brkanac, Sandra ; Pavlović, Iva ; Linić, Ida ; Salopek-Sondi, Branka

engleski

Biochemical response of Arabidopsis thaliana transgenic plants with a disturbed function of enzyme auxin amidohydrolase after salt stress exposure

Biochemical response of Arabidopsis thaliana mutants with impaired function of the enzyme auxin amidohydolase (single ilr1, iar3, and ill2 ; double iar3 ill2 and triple ilr1 iar3 ill2) and the corresponding wild type (wt) of the Wassilewskii ecotype to high salinity (100 mM NaCl) was evaluated after period of 7 days. Auxin amidohydrolases are enzymes that participate in homeostasis of plant hormone auxin by hydrolyzing auxin amide-conjugates and releasing active auxins. The contents of malondialdehyde, proline and ascorbate were determined as well as content of reactive oxygen species (superoxide radical SOˑ and hydrogen peroxide -H2O2) and glutathione using fluorescent probes dihydroethidium (DHE), dichlorodihydrofluorescein diacetate (DCFH-DA) and monochlorobimane (MCB). Salt stress induced significant decrease of ascorbate in ill2 and increase in iar3, iar3 ill2 and iar1 iar3 ill2. Accumulation of proline was significant not only in wt but also in all mutants exposed to salt stress. There was a statistically significant increase in the content of MDA (ilr1, iar3, iar3 ill2 and ilr1 iar3 ill2), glutathione (wt, ill2 and ilr1) and reactive oxygen species (SOˑ ; ilr1 and H2O2 ; ill2) upon salt stress. The highest content of ascorbate, proline and MDA after exposure to salt stress was measured in triple mutant. Based on the obtained results we can conclude that the triple mutant, which has the lowest ability to hydrolyze auxin conjugates, is the most sensitive to salt stress.

Arabidopsis thaliana, transgenic plants, salt stress, biochemical parameters

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Podaci o prilogu

215-216.

2018.

objavljeno

Podaci o matičnoj publikaciji

Book of abstracts of the 13th Croatian biological congress

Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja ; Pavoković, Dubravko ; Kučinić, Mladen

Zagreb: Hrvatsko biološko društvo

Podaci o skupu

13. Hrvatski biološki kongres

poster

19.09.2018-29.09.2018

Poreč, Hrvatska

Povezanost rada

Biologija