engleski

Extraction of polyphenols from sage assisted by high hydrostatic pressure

High hydrostatic pressure extraction (HHPE) was used to obtain polyphenols from sage (Salvia officinalis L.) leaves. In order to determine the optimal conditions for the HHPE extraction of sage polyphenols, the influence of ethanol concentrations (30, 96 %), pressure (300, 450, 600 MPa), extraction time (5, 10, 15 min) and extraction temperature (25, 60 °C) on the composition and mass fraction of polyphenols of wild sage were investigated. Based on the mass fraction of total phenols were selected optimal extraction conditions as follows: 30 % ethanol concentration, 600 MPa of high hydrostatic pressure, extraction time of 5 min and extraction temperature at 60 °C. HHPE reduce the processing time, enhance mass transport phenomena and greater solvent penetration into plant material that provide optimal extraction yields. Using HPLC coupled with UV/PDA, polyphenols isolated from sage including phenolic acid (e.g. rosmarinic acid, rosmarinic acid hexoside, salvianolic acid K, salvianolic acid I, methyl rosmarinate), flavones (e.g. apigenin and luteolin glycosides) and catehines (e.g. catechin, epicatechine). Compared to apigenin and luteolin glycosides and catechines, rosmarinic acid is present at the highest level. The results showed that HHPE can reduce the processing time and provide high extraction yields of polyphenols from plant. The results of this research may lead to finding the possible ecological alternatives for the recovery of polyphenols from sage, enhancing the use of environmental friendly solvents and energy-efficient technologies and promoting the advantages of HHPE for extraction purposes.

extraction, high hydrostatic pressure, sage, Salvia officinalis L., polyphenols

nije evidentirano