Overexpression of YAP1 gene increases the resistance of yeast strains to inhibitors found in lignocellulosic hydrolysates (CROSBI ID 667729)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Štafa, Anamarija ; Djedović, Elvis ; Moguš, Leo ; Žunar, Bojan ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan Krešimir
engleski
Overexpression of YAP1 gene increases the resistance of yeast strains to inhibitors found in lignocellulosic hydrolysates
Due to the constant increase in energy consumption, the production of biofuels from renewable sources, such as lignocellulosic waste, has become more frequent in the last several decades. Lignocellulosic waste consists of cellulose, hemicellulose and lignin and the yeast S. cerevisiae is not able to ferment it directly. Therefore, lignocellulosic waste is usually hydrolysed to release compounds that can be used for fermentation. However, the pretreatment also generates compounds that act as growth and fermentation inhibitors such as acetic and levulinic acid and 2-furaldehyde. In this study, newly constructed intraspecies hybrid diploids, obtained by crossing natural S. cerevisiae isolates, showed higher resistance to acetic and levulinic acid. Selected strains were also transformed with replicative vectors overexpressing yeast genes (e.g. ATR1, CTA1, FLR1, YAP1, ZWF1) encoding proteins involved in a stress response and the overexpression of YAP1 gene further increased the resistance to inhibitors. Therefore, our results suggest that the construction of intraspecies hybrids coupled with genetic engineering is a promising approach for construction of new biotechnologically relevant yeast strains.
gene targeting, targeted chromsome duplication, genome duplication
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
66-66.
2018.
objavljeno
Podaci o matičnoj publikaciji
4th Congress of Croatian Geneticists with International Participation Book of Abstracts
Zagreb: Hrvatsko genetičko društvo
978-953-57128-1-7
Podaci o skupu
4th Congress of Croatian Geneticists with international participation
poster
26.09.2018-29.09.2018
Krk, Hrvatska