Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Cyclodextrin based supramolecular systems functionalized with adamantyl guanidines for gene delivery: preparation, characterization and testing the interaction with DNA (CROSBI ID 667940)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Frkanec, Leo ; Šekutor, Marina ; Frkanec, Ruža Cyclodextrin based supramolecular systems functionalized with adamantyl guanidines for gene delivery: preparation, characterization and testing the interaction with DNA // 8th Regional Biophysics Conference : Book of Abstracts / Majaron, Hana ; Čotar, Petra ; Koren, Monika et al. (ur.). Ljubljana: Slovenian Biophysical Society, 2018. str. 141-141

Podaci o odgovornosti

Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Frkanec, Leo ; Šekutor, Marina ; Frkanec, Ruža

engleski

Cyclodextrin based supramolecular systems functionalized with adamantyl guanidines for gene delivery: preparation, characterization and testing the interaction with DNA

The aim of the present study was preparation and characterization of supramolecular systems with adamantyl guanidine (AG) and probing of their interaction with DNA using fluorescence correlation spectroscopy (FCS). In the continuation of our study dealing with adamantyl guanidines and investigations of lipid-based nanovesicles as drug delivery systems (1, 2) we prepared and characterized functionalized supramolecular systems of adamantyl guanidines AG 1-5 with phosphatidylcholine liposomes, liposomes incorporating amphiphilic -cyclodextrin (-CD) derivative and cyclodextrin vesicles composed only from the amphiphilic -CD derivative. Efficiency of incorporation of AG 1-5 into prepared supramolecular systems was measured spectrophotometrically, and the size and surface charge were determined by dynamic light scattering method. The incorporation of AG 1-5 did not affect the size of prepared supramolecular systems but surface charge was significantly changed. FCS was applied to examine the interactions between functionalized supramolecular systems and the Cy5- fluorescently labelled, double-stranded, 120 bp DNA (DNA120*). The obtained results have clearly demonstrated that the supramolecular systems with entrapped AG 1-4 present guanidinium groups on the surface of vesicles, which leads to interaction with DNA120* via guanidine-phosphate interaction. Preliminary results have shown that prepared functional supramolecular systems strongly bind DNA and as such could be used in gene delivery.

supramolecular systems ; liposomes ; vesicles ; DNA ; fluorescence correlation spectroscopy ; FCS

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

141-141.

2018.

objavljeno

Podaci o matičnoj publikaciji

8th Regional Biophysics Conference : Book of Abstracts

Majaron, Hana ; Čotar, Petra ; Koren, Monika ; Golmajer Zima, Neža

Ljubljana: Slovenian Biophysical Society

978-961-90942-4-2

Podaci o skupu

8th Regional Biophysics Conference (RBC2018)

poster

16.05.2018-20.05.2018

Zreče, Slovenija

Povezanost rada

Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Kemija

Poveznice