engleski

Effect of dietary carob wholemeal on blood parameters in weaned pigs

The aim of this study was to investigate the effect of dietary carob wholemeal (Ceratonia siliqua L.) through monitoring the changes in some blood parameters (RBC, LEU, CD45+, CD4+, CD8+, CD21+) on growth and development of the weaned pigs (cross-breeds of Swedish Landrace and Yorkshire). Thirty pigs were divided into two groups. In control group pigs were fed with a standard feed mixture. In the second, experimental group (EXP), feed mixture was daily enriched with carob wholemeal in a dose of 40 g/kg diet. Both groups were fed ad libitum. At the end of 42-day experiment pigs in EXP groups on average were heavier 23%. The total sugar content, total phenolic content, flavonoids, anthocyanins, tannins and total antioxidant content of carob wholemeal was determined. The carob wholemeal contains high carbohydrate content (73%). The total phenolic content value was 9.98 ± 0.18 mg GAE/g. The total flavonoid value was 6.56 ± 0.19 mg GAE/g, or 65 % of total polyphenols, from which it can be seen that antioxidant activity is correlated to the total phenolic content. The total anthocyanins content was 34.00 ± 0.07 mg CE/g while total tannin content was 658.75 ± 18.75 µg TAE/g. The ABTS method’s values were 89.50 ± 0.17 µmol TE/g sample while antioxidant activity determined with DPPH method was 56.87 ± 0.66 µmol TE/g sample. Carob supplementation did not affect the amount of red blood cells and leucocytes, but did affect the proportions of the proliferation rate of CD45+ lymphoid cells, CD4+ and CD8+ T cells and CD21+ B cells in peripheral blood. Between days 14 and 42 an increase in proportion of CD45+, CD4+, CD8+, CD21+ cells (at p≤0.05 or p≤0.01, respectively) was observed. Dietary supplementation of weaned piglets with 4% carob wholemeal showed advantageous or helpful effect on immunity and productivity of weanlings.

carob, diarrhoea, phytocompounds, immunology, pigs

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