UPLC‐MS2 Profiling of Blackthorn Flower Polyphenols Isolated by Ultrasound‐Assisted Extraction (CROSBI ID 256318)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Elez Garofulić, Ivona ; Zorić, Zoran ; Pedisić, Sandra ; Brnčić, Mladen ; Dragović-Uzelac, Verica
engleski
UPLC‐MS2 Profiling of Blackthorn Flower Polyphenols Isolated by Ultrasound‐Assisted Extraction
Ultrasound-assisted extraction (UAE) was optimized for fast and effective isolation of blackthorn flower polyphenols. The effects of ethanol concentration (50 and 70 %) in extraction solvent, ultrasound amplitude (50, 75 and 100 %) and extraction time (3, 6 and 9 min) were evaluated. UPLC-MS2 was used to determine phenolic profile of blackthorn flowers extracts. A total of 28 different phenolic compounds were identified, belonging to the classes of hydroxycinnamic acids, flavonol glycosides and flavanols. Kaempferol and quercetin derivatives were the most abundant compounds represented by kaempferol- pentoside and rhamnoside in concentrations up to 494.94 and 436.62 mg/100 g and quercetin- pentoside in concentration up to 226.75 mg/100 g. Observed parameters of UAE did not affect the qualitative phenolic composition of blackthorn flower extracts. On the contrary, the concentration of polyphenols increased with higher ethanol concentration in solvent, higher ultrasound amplitude and prolongation of extraction time. The established conditions for optimal extraction of blackthorn flower polyphenols using ultrasound were: 70 % ethanol as extraction solvent, 75 % of ultrasound amplitude and 9 min extraction time, resulting in extract rich in polyphenols with a great potential for use in pharmaceutical and food industry.
blackthorn flowers, polyphenols, RSM, ultrasound-assisted extraction, UPLC-MS2
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Podaci o izdanju
83 (11)
2018.
2782-2789
objavljeno
0022-1147
1750-3841
10.1111/1750-3841.14368
Povezanost rada
Prehrambena tehnologija