DNA methylation in head and neck cancer and oral lesions (CROSBI ID 669143)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Milutin Gašperov, Nina ; Božinović, Ksenija ; Sabol, Ivan ; Grce, Magdalena
engleski
DNA methylation in head and neck cancer and oral lesions
Head and Neck Squamous Cell Carcinoma (HNSCC) is the 7th most frequent malignancy in the world, with a very low 5-year survival. It is known that the main risk factors associated with this carcinoma are excessive tobacco and alcohol consumption together with high-risk types of human papillomavirus (HPV). Still, there is a strong need to find new, good biomarkers of this disease. The most appropriate biomarkers would be those that would point out changes in epithelial cells before carcinoma occurrence. DNA methylation is irreversible change that inhibits gene expression. Altered DNA methylation is one of the possible factors associated with the HNSCC development. The presumption is that epigenetic biomarkers, such as methylated genes could point to changes even before they can be clinically detected. The model on which we explore those changes of DNA methylation are normal oral mucosa tissue, potentially malignant lesions of the oral mucosa, oral lichen planus (OLP) and oral lichenoid lesions (OLL) and HNSCC tissue. OLP and OLL are difficult to distinguish clinically and histopathologically, so early detection and distinguishing by epigenetic tools would be of great importance in further treatment. Herein, we will discuss DNA methylation profiling of different diagnosis by Infinium MethylationEPIC BeadChip array and pyrosequencing methods. The main goal was to reveal DNA methylation differences in normal oral samples, possible in oral lesions, and in HNSCC samples. The results obtained with Infinium MethylationEPIC BeadChip array showed a panel of gene promoters significantly methylated in HNSCC samples in comparison to normal samples, mainly of genes related with receptor and transmembrane functions and transcription activity. Some of them are included in cell cycle, cellular growth, transformation, apoptosis and autophagy. The group of hypomethylated gene promoters in HNSCC, in comparison to normal oral mucosa, and lesions, was mainly involved in immune response and transcriptional regulation.
head and neck cancer ; oral lesions ; DNA methylation
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Podaci o prilogu
27-27.
2018.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Libri oncologici : Croatian journal of oncology
Ozretić, Petar ; Levanat, Sonja
Zagreb: Klinički bolnički centar Sestre milosrdnice
0300-8142
2584-3826
Podaci o skupu
5th Meeting of the Croatian Association for Cancer Research with International Participation: Translating Science to Medicine "Targets and Therapeutics" (HDIR-5)
predavanje
08.11.2018-10.11.2018
Zagreb, Hrvatska