engleski

Combined probiotic effect of S-layer-carrying Lactobacillus brevis SF9B and bacteriocin- expressing Lactobacillus plantarum SF9C strains on faecal microbiota of rats

The aim of this study was to assess the probiotic potential of two Lactobacillus strains naturally occurring as a co-culture in the spontaneous fermented Croatian white cabbage cultivar Varaždinski. The automatic RAST annotation of their whole genome sequences obtained by Illumina MiSeq platform, and tblastn v.2.2.27 comparison of the assembled contigs with the sequences deposited in NCBI, revealed the presence of bacteriocin- encoding genes in the genome of L. plantarum SF9C and surface layer protein (Slp) genes in the genome of Lactobacillus brevis SF9B. Slp may act as an adhesin and contribute to the antimicrobial activity against the pathogens with whom the carrying strain competes for the same adhesion sites. The ability of SF9B cells to compete and exclude Gram-negative pathogens Escherichia coli 3014 and Salmonella enterica serovar Typhimurium FP1 using Caco-2 cell line was hereby confirmed. Moreover, the antimicrobial activity of L. plantarum SF9C against Gram- positive pathogens L. monocytogenes ATCC 19111 and S. aureus 3048 was verified using agar- spot, well diffusion and co- cultivation assay. Moreover, the combined probiotic effect of oral administration of the two Lactobacillus strains on fecal microbiota composition of rats was established.

co-culture ; S-layer ; probiotic ; bacteriocin ; microbiota

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