β-glucan as bio-fixator for aflatoxin M1 (CROSBI ID 670514)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Jakopović, Željko ; Čanak, Iva ; Frece, Jadranka ; Bošnir, Jasna ; Kuharić, Željka ; Pavlek, Željka ; Ivešić, Martina ; Markov, Ksenija
engleski
β-glucan as bio-fixator for aflatoxin M1
Aflatoxin M1 (AFM1), a hydroxylated metabolite of aflatoxin B1, is relatively stable molecule in raw and processed dairy products and cannot be inactivated by heat treatments such as pasteurization or sterilization. Due to the fact that presence of AFM1 in milk can be dangerous to human health and presents an enormous economic problem, there is an increasing emphasis on the development of methods for AFM1 reduction. One of the strategies is the use of various types of mycofixators (alumino-silicates, clay, zeolites etc.) that have the ability to bind mycotoxins and are removed from food after use. Despite the use of mycofixators in feedstuffs, AFM1 contaminated milk occurs. Therefore, the aim of this work was to determine the binding parameters of AFM1 in milk by β-glucan isolated from oats and yeast. Several concentrations of β-glucan were incubated in AFM1-contaminated milk during 24 hours. Binding efficiency was determined by quantifying the unbound AFM1 using (UP)LC-MS/MS method. Depending on these adsorbents the percentages of bound AFM1 varied from as low as 8, 2 % to as high as 65 %. Results also show noticeable nonlinearity during 24 hours when binding AFM1 with β-glucan from yeast compared to β-glucan from oats, which can be consequence of the reversible reaction of AFM1- β-glucan complex formation. β-glucan from oats has shown slightly better ability (65%) to bind AFM1 from artificailly contaminated milk compared to β-glucan isolated from yeast (63, 6%). These differences in binding ability are most likely due to the different structure and molecular branching of β-glucans.
AFM1 ; β-glucan ; biological method ; milk ; (UP)LC-MS/MS
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Podaci o prilogu
56-56.
2018.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju
Zagreb: Institut za medicinska istraživanja i medicinu rada
0004-1254
1848-6312
Podaci o skupu
2. međunarodni kongres o sigurnosti i kvaliteti hrane = 2nd International Congress on Food Safety and Quality
poster
13.11.2018-16.11.2018
Opatija, Hrvatska