A new role of RecA protein in Escherichia coli? (CROSBI ID 670630)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Đermić, Edyta ; Zahradka, Davor ; Vujaklija, Dušica ; Đermić, Damir
engleski
A new role of RecA protein in Escherichia coli?
The ubiquitous and evolutionary conserved RecA protein binds to single-stranded DNA (ssDNA), producing a nucleoprotein filament. RecA has three known roles in E. coli: a) RecA nucleoprotein filament enables repair of DNA double-strand breaks (DSBs) and exchange of similar DNA molecules by promoting homologous recombination ; b) RecA nucleofilament facilitates autocleavage of LexA repressor, hence enabling induction of SOS response ; c) during SOS induction, RecA activates a mutagenic DNA polymerase V. Another role of RecA exists in E. coli, namely RecA inhibits DNA degradation. However, no relation of the latter RecA function to the three established ones has been explored previously. Here, we introduced DSBs (by gamma-irradiation) into E. coli chromosome labelled by [3H]thymidine and monitored its degradation. We have shown that RecA inhibition of DNA degradation depends on RecA’s concentration and its rate of association with ssDNA. Also, our results indicate that RecA suppression of DNA degradation is separable from the other three roles of RecA, thus suggesting it represents a novel RecA function in E. coli, namely regulation of DSB processing.
Escherichia coli ; RecA protein ; DNA double-strand breaks ; DNA degradation
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Podaci o prilogu
44-44.
2018.
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objavljeno
978-953-57128-1-7
Podaci o matičnoj publikaciji
4th Congress of Croatian Geneticists with International Participation : Book of Abstracts
Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina
Zagreb: Hrvatsko genetičko društvo
Podaci o skupu
4th Congress of Croatian Geneticists with international participation
poster
26.09.2018-29.09.2018
Krk, Hrvatska