Toxicology and antitumor evaluation of two novel monomethine cyanine derivatives in vivo (CROSBI ID 671346)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Mišković, Katarina ; Belovari, Tatjana ; Rajc, Jasmina ; Šerić, Vatroslav ; Stojković, Ranko ; Piantanida, Ivo ; Baus Lončar, Mirela ; Glavaš-Obrovac, Ljubica
engleski
Toxicology and antitumor evaluation of two novel monomethine cyanine derivatives in vivo
The area of small, biological active molecules that interact with specific cell compartment are in focus of today’s science. Small molecules with low toxicity and specific mode of interaction are interesting in the area of molecular therapy especially for cancer treatment and future of personalised medicine. One, very interesting biologically active group of small molecules are monomethine cyanine dyes (MCDs) with preferable binding mechanism in to a minor groove of DNA. In this study we evaluate toxicological and antitumor effect of two, new monomethine cyanine derivatives designated as monomethine cyanine derivatives no. 4 and no.8. Highly inbreeded hybrid mouse WT strain created as combination of 129/Sv and C57BL/6j were used for acute and chronic toxicology study and BalbC female mice were used for antitumor study. Four mice for each sex were used in experimental groups. Haematological (E, L, Hg, HE, MCV, MCV, MCHC, Plt ) and biochemical (AST, ALT, AP, LDH, glucose, urea, creatinine, Na, K) parameters, histomorfological analysis of body organs (brain, hart, spleen, bone, stomach, intestine, colon, kidney, liver, lungs) and size of grown tumour are measured as tool for in vivo effect. Obtained results are analysed by multifactorial variance analysis (MANOVA) and Fisher LSD test in STATISTICA 8.0 program. One time application (7mg/kg) with MCD 4 in evaluation of acute toxicity resulted with increased levels of AST, ALT and LDH and decreased glucose. Chronic toxicity (3x7mg/kg) because of the prolonged exposure and multiple MCD 4 application did not show significant change on the male mice. MCD 4 applied to the female mice raised levels of AST, ALT and LDH and decreases ALP. Results points to enhanced sensibility of female mice compared to males in prolonged time of exposure to MCD 4. Chronic toxicity caused by the MCD 8 did not point to any significantly altered biochemical parameter no matter the sex of the mouse. Results pointed to stronger MCD 4 toxic effect in chronic exposure to female in comparison to male mice. There were no significant suppression of implanted tumour regardless to applied concentration of tested derivatives.
antitumor evaluation ; monomethine cyanine derivatives
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Podaci o prilogu
255-255.
2017.
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objavljeno
978-953-57695-2-1
Podaci o matičnoj publikaciji
10th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine : Program and abstracts
Primorac, Dragan ; Schanfiled, Moses ; Vuk-Pavlović, Stanimir ; Kayse, Manfred ; Ordog, Tamas
Zagreb: International Society for Applied Biological Sciences (ISABS)
Podaci o skupu
10th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine
poster
19.06.2017-24.06.2017
Dubrovnik, Hrvatska