engleski

Untargeted metabolomics approach in the study of posttraumatic stress disorder

Posttraumatic stress disorder (PTSD) is a common, prevalent, severe, disabling and debilitating mental disorder developing after traumatic exposure. The exact biological factors, associated with vulnerability or resilience to develop PTSD after traumatic exposure, are still not well known. In this research we sought to investigate a metabolic profile of patients with combat related PTSD. Study included 100 plasma samples, 50 from PTSD subjects and 50 from matching healthy control (HC) subjects. Samples were collected within the project „Genomic and glycomic biomarkers for PTSD” supported by Croatian Science Foundation. Plasma samples were analyzed by a Liquid Chromatography (Agilent 1200) coupled to Mass Spectrometry (LC - QTOF-MS (Agilent 6520) in positive and negative ionization mode. Primary analysis was followed by tandem LC-MS experiments in order to identify significant compounds. After multivariate (principal component analysis, partial least square-discriminant analysis) and univariate (Student’s t-test, Mann-Whitney U test) statistical analysis, 25 significant chemical signals were found in positive and 35 in negative LC-MS mode. A trend of increased levels of different types of glycerophospholipids and decreased levels of different types of carnitines and bile acids was found in PTSD subjects when compared to HC subjects. This preliminary study could contribute to better understanding of PTSD etiology, but further research has to be carried out to validate current results.

Metabolomics ; PTSD ; LC-MS

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