Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Application of 4-amino-N-adamantylphthalimide solvatochromic dye for fluorescence microscopy in selective visualization of lipid droplets and mitochondria (CROSBI ID 259777)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Benčić, Patricia ; Mandić, Leo ; Džeba, Iva ; Tartaro Bujak, Ivana ; Biczók, László ; Mihaljević, Branka ; Mlinarić-Majerski, Kata ; Weber, Igor ; Kralj, Marijeta ; Basarić, Nikola Application of 4-amino-N-adamantylphthalimide solvatochromic dye for fluorescence microscopy in selective visualization of lipid droplets and mitochondria // Sensors and actuators. B, Chemical, 286 (2019), x; 52-61. doi: 10.1016/j.snb.2019.01.102

Podaci o odgovornosti

Benčić, Patricia ; Mandić, Leo ; Džeba, Iva ; Tartaro Bujak, Ivana ; Biczók, László ; Mihaljević, Branka ; Mlinarić-Majerski, Kata ; Weber, Igor ; Kralj, Marijeta ; Basarić, Nikola

engleski

Application of 4-amino-N-adamantylphthalimide solvatochromic dye for fluorescence microscopy in selective visualization of lipid droplets and mitochondria

4-Amino-N-adamantylphthalimide (1) is a dye with moderate absorptivity (in CH3CN ε363 = 4200 M-1 cm-1) and high quantum yield of fluorescence (ΦF = 0.15-0.80) that exhibits fluorosolvatochromic properties. The dye can be excited at 405 nm and the position of fluorescence maximum and the Stokes shift are well correlated with the ET(30) parameter. The excitation in the near-visible part of the spectrum and low cytotoxicity allow use of the dye in live cell microscopy. Due to its amphiphilic character, the dye stains artificial membranes in liposomes. Using confocal microscopy on two human cancer cell lines, we have shown that 1 stains primarily intracellular lipid droplets. Colocalization experiments with different organelle markers indicated that 1 additionally stains mitochondrial membranes. The fluorosolvatochromism of 1 allows the simultaneous visualization of mitochondria and intracellular lipid droplets in two separate emission channels, which has a potential use in cells and tissues exhibiting intense oxidative metabolism of lipids.

aminophthalimide, biological membranes, fluorescent dyes, confocal fluorescence microscopy, nonpolar lipids

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

286 (x)

2019.

52-61

objavljeno

0925-4005

1873-3077

10.1016/j.snb.2019.01.102

Povezanost rada

Kemija, Temeljne medicinske znanosti

Poveznice
Indeksiranost