engleski

Effect of liposome composition on calcium phosphate precipitation

Biomineralization is formation of hard tissues in organisms. From physical-chemical point of view it can be considered as a precipitation/crystallization process of inorganic minerals within the organic matrix and/or confined environments. The liposomes closed lipid bilayers, are typical experimental model of confined environment in which biomineralization can occur. The influence of lipid molecules’ structure or their chemical composition and charge (anionic, cationic, zwitterion), on biomineralization is still not fully understand. The aim of this study is to investigate the influence of three lipids (dimyristoylphosphatidylcholine, DMPC, dimyristoylphosphatidylserine, DMPS and phosphatidylcholine, EPC) with different charge of their polar heads, on CaP precipitation. Two precipitation strategies were investigated: fusion of liposomes loaded with constitutive ions (Ca2+ and HPO42–) and spontaneous precipitation of CaP in the presence of liposomes. DMPS exhibited the strongest influence on the precipitation and transformation of the CaP, in both precipitation systems. The obtained results may contribute to the understanding of the general mechanism of inorganic–organic interactions underlying the biomineralization processes, as well as for materials science.

calcium phosphates ; liposomes

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