engleski

HLA-B27 subtypes in Croatian patients with ankylosing spondylitis

A total of 119 unrealted B27+ AS patients and 165 B27+ healthy controls were included in this study. For all individuals HLA genotyping was performed using segregation data of parental haplotypes. The AS patients were clinically diagnosed according to the New York criteria of 1986. HLA-A and -B typing was performed using the standard MLCT test on local trays. PCR-SSP typing for the B-27 subtypes was performed using Dynal HLA-B27 high resolution kit. Present study did not demonstarte any of B27 alleles as susceptible or protective genetic marker for AS in the Croatian population and it fits into the frame of the oldness of the association between AS and B27. AS appears to be older than the origin of the B27 subtypes.

HLA-B27 alleles; ankylosing spondylitis; population study

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