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Application of biopolymer microcapsules/ microspheres loaded with Trichoderma viride and calcium/copper ions during a hydroponic cultivation of lettuce

Encapsulation of bioactive agents such as fungi and agrochemicals is a promising way for nutrients delivery for a sustainable and safe plant production. Encapsulation of bioactive agents can slow and control the release of active compounds, therefore more efficient use of agrochemicals with better safety and environmental protection. Trichoderma viride has shown the ability to enhance plant growth and its productivity. The dual roles of antagonistic activity against plant pathogens and promotion of soil fertility make Trichoderma species as a promising alternative to standard plant protection and nutrition technologies. Based on our previous research, microparticles containing different combinations of Ca2+or Cu2+ ions, T. viride, alginate, and additional chitosan coating were optimized and applied in the hydroponic cultivation of lettuce (Lactuca sativa L.). Obtained ethanolic extracts of fresh lettuce were analyzed in terms of biologically active compounds. Compared to the control, results revealed a positive influence of microparticles on obtained biomass yield, rosette diameter, and height of lettuce as well as the significant impact on bioactive compounds content and antioxidant activity of ethanolic lettuce extracts. Encapsulation of biological and chemical agents in biopolymeric microparticles has shown to be a sustainable, environmentally friendly, rapid, convenient, economical and efficient delivery of active agents for plant nutrition.

encapsulation, hydroponics, chemical agents, Trichoderma viride, microcapsules/ microspheres

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