Interference sample as a source of preanalytical variablity: results of Croatian preanalytical External Quality Assessment CROQALM program (CROSBI ID 673970)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Nikolac Gabaj, Nora ; Ćelap, Ivana ; Leniček Krleža, Jasna
engleski
Interference sample as a source of preanalytical variablity: results of Croatian preanalytical External Quality Assessment CROQALM program
BACKGROUND-AIM: The aim of this study is to present results of distributing icteric sample within Croatian preanalytical External Quality Assessment (EQA) provider CROQALM. METHODS: All Croatian laboratories (N = 194) were invited to participate in free-of-charge preanalytical EQA during 2018. In March (Sample 1) and September (Sample 3) serum sample for clinical chemistry, while in June (Sample 2) citrate plasma for coagulation were distributed. Samples were prepared as follows: leftover serum/citrate plasma samples without any visible interference were collected, mixed, immediately frozen at -20 °C for 48 hours, thawed and filtered once using filter paper. Pools were spiked with powdered bilirubin (Sigma-Aldrich, Missouri, USA) in order to create icteric samples. Samples were aliquoted and sent out to laboratories within 48 hours. Participants were asked to evaluate the degree of interference. Responses were collected via service SurveyMonkey (California, USA). Variables were compared using Chi-square test in MedCalc (v12.7.2.0, Ostend, Belgium). P < 0.05 was considered statistically significant. RESULTS: Majority of laboratories evaluate icteria visually (108/161, 67.1% ; 140/148, 94.6% ; 101/156, 64.7% in cycles 1-3, respectively) with lower representation of automatic evaluation in coagulation (cycle 2 ; P < 0.001). When asked to visually evaluate the sample, 109/157, 69.4% ; 103/144, 71.5% ; 101/153, 66% gave the preferred answer (“icteric sample”), no differences between cycles were observed (P = 0.450). Out of laboratories using automatic evaluation of icteria, significantly more expressed index quantitatively in cycle 3 vs. 1. (78.8% vs. 42.2% ; P < 0.001). No laboratory provided data on automatic index in coagulation cycle. 2/3 of laboratories express I index in μmol/L. CONCLUSIONS: Large variability between Croatian laboratories was observed in management of icteric sample, with automatic evaluation being more implemented in clinical chemistry than in coagulation.
preanalytical phase, external quality assessment, interference, icteria
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Podaci o prilogu
eA72-eA73.
2019.
nije evidentirano
objavljeno
10.1515/cclm-2019-0104
Podaci o matičnoj publikaciji
Clinical chemistry and laboratory medicine
Plebani, Mario
Berlin: Walter de Gruyter
1434-6621
Podaci o skupu
5th EFLM Conference on Preanalytical Phase
poster
22.03.2019-23.03.2019
Zagreb, Hrvatska
Povezanost rada
Kliničke medicinske znanosti