Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

12-plex highly polymorphic microsatellite marker set for parentage analysis in Banija spotted pigs (CROSBI ID 262786)

Prilog u časopisu | kratko priopćenje | međunarodna recenzija

Margeta, Polona ; Škorput, Dubravko ; Šalamon, Dragica ; Menčik, Sven ; Gvozdanović, Kristina ; Karolyi, Danijel ; Luković, Zoran ; Salajpal, Krešimir 12-plex highly polymorphic microsatellite marker set for parentage analysis in Banija spotted pigs // Journal of Central European agriculture, 20 (2019), 1; 50-54. doi: 10.5513/JCEA01/20.1.2407

Podaci o odgovornosti

Margeta, Polona ; Škorput, Dubravko ; Šalamon, Dragica ; Menčik, Sven ; Gvozdanović, Kristina ; Karolyi, Danijel ; Luković, Zoran ; Salajpal, Krešimir

engleski

12-plex highly polymorphic microsatellite marker set for parentage analysis in Banija spotted pigs

Microsatellites (MS) have been, for the last two decades, widely used for parentage analysis in all types of livestock, including pigs. Conservation efforts on Banija spotted pigs included genetic characterization of the breed with MS markers. Since recent comparison of pedigree and MS data revealed some inconsistencies, the aim of this study was to develop a set of highly polymorphic and heterozygous MS markers, which could be used for parentage analysis and to prevent pedigree errors. 12 MS markers with the polymorphic information content (PIC) above 0.62 were chosen and combined into a single multiplex polymerase chain reaction (PCR). The combined non-exclusion probability for one candidate parent (NE-1P) was 0.00246149, the combined NE-1P given the genotype of a known parent of the opposite sex (NE-2P) was 0.00003739 and combined non- exclusion probability for a candidate parent pair (NE-PP) was 0.00000003. Due to a high information content of selected MS markers it was possible to obtain high accuracy in parentage assignment, which was confirmed by analyzing actual data with known genetic relationships

Banija spotted pig, microsatellite multiplex, parentage analysis

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

20 (1)

2019.

50-54

objavljeno

1332-9049

10.5513/JCEA01/20.1.2407

Povezanost rada

Biologija, Biotehnologija, Poljoprivreda (agronomija), Veterinarska medicina

Poveznice
Indeksiranost