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Natural zeolite clinoptilolite increases the concentrations of sphingoid bases in the yeast Yarrowia lipolytica (CROSBI ID 97486)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Bauman, Mirela ; Mesarić, Marko ; Ribar, Slavica ; Marić, Vladimir ; Tudja, Marijan Natural zeolite clinoptilolite increases the concentrations of sphingoid bases in the yeast Yarrowia lipolytica // Journal of Basic Microbiology, 41 (2001), 1; 7-16-x

Podaci o odgovornosti

Bauman, Mirela ; Mesarić, Marko ; Ribar, Slavica ; Marić, Vladimir ; Tudja, Marijan

engleski

Natural zeolite clinoptilolite increases the concentrations of sphingoid bases in the yeast Yarrowia lipolytica

In the present paper, we studied the effect of natural zeolite clinoptilolite on sphingolipid mteabolism in the yeast Yarrowia lipolytica. We also investigated if zeolite addition had any impact on cell shape and size, as well as on the pH alterations during the culture growth. High performance liquid chromatography analysis of sphingoid bases obtained by acid hydrolysis of complex sphingolipids from Y. lipolytica showed that their concentrations markedly rose upon the zeolite addition. The largest increase among the identified molecular species of sphingoid bases was seen in C18 phytosphingosine, whose levels rose 6.2-fold and 22.3-fold after culturing cells for 24 and 36 hours respectively in the presence of finely ground zeolite. pH measurements of the culture medium showed a similarity between pH profiles of control and zeolite-supplemented cells, suggesting that ion-exchange capacity was not probably responsible for the observed change in sphingolipid metabolism. Scanning electron microscopy revealed that zeolite affected cell size and shape. Y. lipolytica cells grown in the absence of zeolite were oval-shaped with an average cell size of 0.7-2.7 mm, whereas when cultured with zeolite, they were round-shaped and larger, having an average cell size of 1.3-2.9 mm.

yeast; sphingolipids; sphingoid bases; natural zeolite

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Podaci o izdanju

41 (1)

2001.

7-16-x

objavljeno

0233-111X

Povezanost rada

Prehrambena tehnologija, Biotehnologija

Indeksiranost