engleski

Molecular analysis of the most frequent HLA-B group alleles in the croatian population

The HLA-B5, -B7, -B8, -B12, and -B35 are five of the most frequent antigens in Croatians. These alleles are very hetrogenous HLA-B specificity with many subtypes which cannot be distinguish by serology and only few of them are revealed by biochemical methods. In order to define subtypes of the above HLA-B antigens in our population, we have studied 156 healthy, unrelated donors as a part of the the anthropology component of the 13th International Histocompatibility Workshop (13thIHW). All donors were from wider area of the Croatian capital city, Zagreb. Genomic DNA was isolated from peripheral blood leukocytes by a standard salting-out method. All samples were amplified using PCR and tested with Reverse Line Strip method according protocols of 13thIHW. Among B*51 alleles, B*5101 was predominat with 11.9% frequency while B*5102 alleles occured twice (0.6%). Allele B*0702 (9.9%) was only observed allele between 16 different B*07 subtypes. Four different B*35 alleles were observed (B*3501-5.6%, B*3503-2.9%, B*3502-2.9%, B*3520-1.3%, while B*3508 was present once (0.3%). In the group of B8 alleles, B*0801 was most frequent (2.2%), but we also observed B*0804 (1.3%) and B*0805 (0.6%). B*4402 (6.1%) occured three times more than B*4403, and one individuals had B*4407 (0.3%). Allele B*4501 was present twice (0.6%). Furthermore, analysis of haplotypic associations showed that A*0201-B*5101 (2.7%), A*0301-B*0702 (3.7%), A*0201-B*3501 (2.9%), A*0101-B*0801 (3.5%) and A*0201-B*4402 (1.6%) were most frequent. When HLA-A9-B12 haplotypic associations were analysed, A*2402 was associated with B*4402, while B*4403 was always associated with A*2302. This molecular data of the most frequent HLA-B alleles and their haplotypic associations will be helpful for further anthropology studies as well as for comparing findings for our population with results from other Caucasoid populations. It will also serve as reference for donor/recipient matching in organ and bone marrow transplantation program.

HLA-B; molecular analysis; population data

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