Development and application of a DNA microarray for gene expression profiling in captive Atlantic bluefin tuna (Thunnus thynnus L., 1758) (CROSBI ID 426298)
Ocjenski rad | doktorska disertacija
Podaci o odgovornosti
Trumbić, Željka
Mladineo, Ivona ; Taggart, John
engleski
Development and application of a DNA microarray for gene expression profiling in captive Atlantic bluefin tuna (Thunnus thynnus L., 1758)
Atlantic bluefin tuna Thunnus thynnus is migratory fish with distinct physiology and high economic value. In this study mixed-tissue normalized cDNA library was constructed, pyrosequenced, assembled and annotated. It was used as a template for construction of T. thynnus-specific DNA microarray with 15 000 probes used to infer tissue-specific gene expression profiles. The relationship between bluefin as a host and digenean trematode Didymosulcus katsuwonicola was further investigated employing DNA microarray and transmission electron microscopy.
Thunnus thynnus ; Didymosulcus katsuwonicola ; transcriptome sequencing ; DNA microarray ; Trematoda ; ultrastructure
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Podaci o izdanju
115
15.09.2015.
obranjeno
Podaci o ustanovi koja je dodijelila akademski stupanj
Sveučilište Josipa Jurja Strossmayera u Osijeku; Sveučilište u Dubrovniku; Institut Ruđer Bošković
Osijek