Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats (CROSBI ID 486980)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Mršić Pelčić, Jasenka ; Pelčić, Goran ; Vitezić, Dinko ; Župan, Gordana ; Simonić, Ante The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats // Periodicum biologorum (ISSN 0031-5362), Vol 103, Supp 1. Zagreb: Hrvatsko prirodoslovno društvo, 2001. str. 104-x

Podaci o odgovornosti

Mršić Pelčić, Jasenka ; Pelčić, Goran ; Vitezić, Dinko ; Župan, Gordana ; Simonić, Ante

engleski

The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats

Introduction: The aim of our study was to examine the influence of hyperbaric oxygenation (HBO) on Na+, K+-ATPase activity in optic nerve of ischemic rats. Materials and Methods: Animals: Hannover-Wistar rats, weighing 250 g. Models: Global cerebral ischemia: vertebral arteries were occluded by electrocautery at the level of the first cervical vertebra.The day after, the common carotid arteries were exposed and a forebrain ischemia was produced by tightening the carotid artery clips for 20 min. Na+, K+-ATPase assay: Na+, K+-ATPase activity was determined spectrophotometrically by modification of the method described by Stefanovic et al (1974). Hyperbaric oxygenation: Animals have been exposed to HBO during seven consecutive days, at 222.86 kPa pressure of 99.5% oxygen, lasting 60 min. Procedure: Animals were exposed to global cerebral ischemia and after different reperfusion period (immediately after ischemia, 30 min, 1, 2, 24, 48, 72 and 168 hrs), Na+, K+ -ATPase activity was measured. A part of ischemic animals were exposed to HBO after reperfusion. Results: We found significant decrease in Na+, K+-ATPase activity in periods from 30 min to 7 days after ischemia. The most profound decrease was registered in 24 hrs reperfusion time. Treatment with HBO prevented enzymatic inhibition in animals exposed to hyperbaric oxygen in a period up to 24 hrs after ischemic injury. Conclusions: Decrease in Na+, K+-ATPase activity was evident in ischemic animals exposed to different reperfusion intervals. Treatment with HBO maintained enzymatic activity up to control levels, and exerted neuroprotective effect.

Brain ischemia; Hyperbaric oxygenation; Na+; K+-ATPase activity; Optic nerve; Rats

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

104-x.

2001.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Periodicum biologorum (ISSN 0031-5362), Vol 103, Supp 1

Zagreb: Hrvatsko prirodoslovno društvo

Podaci o skupu

3rd Croatian Congress Of Pharmacology with international participation

poster

01.09.2001-04.09.2001

Zagreb, Hrvatska

Povezanost rada

Temeljne medicinske znanosti