The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats (CROSBI ID 486980)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Mršić Pelčić, Jasenka ; Pelčić, Goran ; Vitezić, Dinko ; Župan, Gordana ; Simonić, Ante
engleski
The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats
Introduction: The aim of our study was to examine the influence of hyperbaric oxygenation (HBO) on Na+, K+-ATPase activity in optic nerve of ischemic rats. Materials and Methods: Animals: Hannover-Wistar rats, weighing 250 g. Models: Global cerebral ischemia: vertebral arteries were occluded by electrocautery at the level of the first cervical vertebra.The day after, the common carotid arteries were exposed and a forebrain ischemia was produced by tightening the carotid artery clips for 20 min. Na+, K+-ATPase assay: Na+, K+-ATPase activity was determined spectrophotometrically by modification of the method described by Stefanovic et al (1974). Hyperbaric oxygenation: Animals have been exposed to HBO during seven consecutive days, at 222.86 kPa pressure of 99.5% oxygen, lasting 60 min. Procedure: Animals were exposed to global cerebral ischemia and after different reperfusion period (immediately after ischemia, 30 min, 1, 2, 24, 48, 72 and 168 hrs), Na+, K+ -ATPase activity was measured. A part of ischemic animals were exposed to HBO after reperfusion. Results: We found significant decrease in Na+, K+-ATPase activity in periods from 30 min to 7 days after ischemia. The most profound decrease was registered in 24 hrs reperfusion time. Treatment with HBO prevented enzymatic inhibition in animals exposed to hyperbaric oxygen in a period up to 24 hrs after ischemic injury. Conclusions: Decrease in Na+, K+-ATPase activity was evident in ischemic animals exposed to different reperfusion intervals. Treatment with HBO maintained enzymatic activity up to control levels, and exerted neuroprotective effect.
Brain ischemia; Hyperbaric oxygenation; Na+; K+-ATPase activity; Optic nerve; Rats
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Podaci o prilogu
104-x.
2001.
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objavljeno
Podaci o matičnoj publikaciji
Periodicum biologorum (ISSN 0031-5362), Vol 103, Supp 1
Zagreb: Hrvatsko prirodoslovno društvo
Podaci o skupu
3rd Croatian Congress Of Pharmacology with international participation
poster
01.09.2001-04.09.2001
Zagreb, Hrvatska