engleski

Escherichia coli metabolic network modelling

This work presents mathematical model of Escherichia coli metabolism based on kinetic data and annotated genome information by J.S. Edwards(1). Published reaction rates and kinetic parameters are used for derivation of mass balances for the key metabolic networks. The balances are given in dynamic form, which enables simulation of transient metabolic behaviour under varying experimental conditions. Applied is stoichiometric analysis of reaction rate constraints. The computer model is based on a set of stiff nonlinear ordinary differential equations (ODE) with specified initial conditions. Applied is NDSolve numerical integration program for evaluation of the model equations. The results are presented in graphical form enabling visualisation of key metabolic pathways. The results are discussed with various published data from literature

Escherichia coli; metabolic engineering; computer simulation

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