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Bibliographic record number: 324184


Authors: Krpan, Marina; Hruškar, Mirjana; Marković, Ksenija; Vahčić, Nada
Title: Development and validation of the HPLC method for determination of nucleotides in infant formulas
Source: Annals of Nutrition & Metabolism / Guilloteau, Paul ; Lemonnier, Daniel ; Lairon, Denis (ed). - Basel : Karger AG , 2007. 170-171 (ISBN: 978-3-8055-8335-0).
ISSN: 0250-6807
Meeting: 10th European Nutrition Conference
Location and date: Pariz, Francuska, 10-13.07.2007.
Keywords: HPLC method; Infant formulas; Nucleotides; Validation
Researchers have established that human milk is the only source of nucleotides for infant during first months of life. Dietary nucleotides seem to influence several aspects of neonatal development such as modulate lipoprotein metabolism, modify the composition of the intestinal microflora, improve gastrointestinal tract, repair after damage and participate in the immunity response mediated by T-cells. Since, the infant formulas supplemented with nucleotides are developed. The aim of these investigations was development of the method for determination of 5’ -AMP, 5’ -CMP, 5’ -GMP, 5’ -UMP and 5’ -IMP in infant formulas. Because the chosen methods can influence on the quality of achieved results, in this work the validation of HPLC methods has been studied. A method for the determination of 5’ -AMP, 5’ -CMP, 5’ -GMP, 5’ -UMP and 5’ -IMP in infant formulas was optimized and its performance evaluated. Method is based on deproteinisation of samples and direct analysis by ion-pair HPLC using Supelcolsil LC-18-T column, followed by diode-array detection. During validation process the following parameters were examined: selectivity, precision, accuracy, linearity and robust. The obtained results are summarised and showed that this method is specificity. The precision of the method is evaluated and a coefficient of variation (CV %) is less than 3 %. Validation of the proposed method was carried out by standard additions method, with recoveries of 100 ± 3 ; the relative standard deviation was ≤ 3. For linearity the correlation coefficient was r2 ≥ 0, 999. Solutions are stable during 30 hours and in range of method robustness. Validation of HPLC method for determination of nucleotides has showed that developed analytical method is acceptable for its intended purpose in defined labour conditions.
Citation databases: Current Contents Connect (CCC)
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Type of meeting: Poster
Type of presentation in a journal: Abstract
Type of peer-review: International peer-review
Project / theme: 058-0580696-2808
Original language: ENG
Category: Znanstveni
Research fields:
Contrib. to CROSBI by: Nada Vahcic (, 4. Ožu. 2008. u 12:23 sati

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