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Bibliographic record number: 488214

Journal

Authors: Pletikapić, Galja; Mišić Radić, Tea; Hozić Zimmermann, Amela; Svetličić, Vesna; Pfannkuchen, Martin; Marić, Daniela; Godrijan, Jelena; Žutić, Vera
Title: AFM imaging of extracellular polymer release by marine diatom Cylindrotheca closterium (Ehrenberg) Reiman & J.C. Lewin
Source: Journal of molecular recognition (0952-3499) 24 (2011), 3; 436-445
Paper type: article
Keywords: marine diatom; Cylindrotheca closterium; Ceratoneis closterium; extracellular polymer substance; polysaccharides; atomic force microscopy; Adriatic Sea
Abstract:
Extracellular polysaccharide production by marine diatoms is a significant route by which photosynthetically produced organic carbon enters the trophic web and may influence the physical environment in the sea. This study highlights the capacity of AFM for investigating diatom extracellular polysaccharides with a subnanometer resolution. Here we address a ubiquitous marine diatom Cylindrotheca closterium, isolated from the Northern Adriatic seawater, and its extracellular polymeric substance (EPS) at a single cell level. We applied a simple procedure for AFM imaging of diatom cells on mica under ambient conditions (in air) to achieve visualization of their EPS with molecular resolution. The EPS represents a web of polysaccharide fibrils with two types of cross-linking: fibrils association forming junction zones, and fibril-globule interconnections with globules connecting two or more fibrils. The fibril heights were 0.4-2.6 nm while globules height was in the range of 3-12 nm. Polymer networks of native gel samples from the Northern Adriatic and the network formed by polysaccharides extracted from the C. closterium culture share the same features regarding the fibril heights, pore openings and the mode of fibril association, proving that the macroscopic gel phase in the Northern Adriatic can be formed directly by the self-assembly of diatom released polysaccharide fibrils.
Project / theme: 098-0982934-2744
Original language: ENG
Citation databases: Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Category: Znanstveni
Research fields:
Geology,Chemistry,Biology
URL: http://onlinelibrary.wiley.com/doi/10.1002/jmr.1114/abstract
Broj citata:
Altmetric:
DOI: 10.1002/jmr.1114
URL cjelovitog rada:
Google Scholar: AFM imaging of extracellular polymer release by marine diatom Cylindrotheca closterium (Ehrenberg) Reiman & J.C. Lewin
Contrib. to CROSBI by: tmisic@irb.hr (tmisic@irb.hr), 26. Lis. 2010. u 11:24 sati



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