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Bibliographic record number: 543363

Journal

Authors: Stanzer, Damir; Mrvčić, Jasna; Križanović, Stela; Stehlik-Tomas, Vesna; Grba, Slobodan
Title: Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli
Source: Chemical and biochemical engeenering quartely (0352-9568) 25 (2011), 4; 513-517
Paper type: article
Keywords: D-Amino acid oxidase; Trigonopsis variabilis; Escherichia coli; D-methionine
Abstract:
D-Amino acid oxidase is a key enzyme in the production of semisynthetic cephalosporins. The Trigonopsis variabilis D-amino acid oxidase with 12- amino-acid peptide at N-terminus was expressed in Escherichia coli BL21(DE3), in a series of short fed-batch cultivations. The strategy of gradual induction and gradual feeding with D-methionine during expression resulted in active enzyme production twice as high as that in which the inducer and methionine were added all at once at the expression start. In optimal conditions during 14 h of aerobic cultivation, 350 mg/L of active enzyme was produced.
Project / theme: 058-0580477-0374, 058-0583444-3483
Original language: ENG
Citation databases: Current Contents Connect (CCC)
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Category: Znanstveni
Research fields:
Biotechnology,Food processing technology
URL: http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=113556
URL cjelovitog rada:
Google Scholar: Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli
Contrib. to CROSBI by: jmrvcic@pbf.hr (jmrvcic@pbf.hr), 17. Stu. 2011. u 14:19 sati



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