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Bibliographic record number: 676743


Authors: Butorac, Ana; Cindrić, Mario; Mrvčić, Jasna; Bačun-Družina; Višnja
Title: Evolution of bacterial population: The metabolic engineering of Lactobacillus brevis
Source: The 2th International Symposium "Vera Johanides" / Kniewald ; Zlatko (ed). - Zagreb : D.S.M-grafika d.o.o , 2013. 122-122 (ISBN: 978-953-7076-21-4).
Meeting: The 2th International Symposium "Vera Johanides"
Location and date: Zagreb, Hrvatska, 10-11.06.2013.
Keywords: Lactobacillus; evolution; long term starvation
Lactic acid bacteria in particular Lactobacilli are widely used in food and pharmaceutical industries. Lactobacillus brevis occupies a diverse range of environmental niches and has various metabolic capacities. Its genomic islands are consisted of numerous functional gene cassettes, especially for carbohydrates utilization, which can be mobilized, shuffled, deleted or substituted in response to niche demands. We used laboratory evolution experiments to explore bacterial adaptation under well-defined selective pressure of prolonged stationary phase growth. Genomics analyses of L. brevis after long term-starvation indicated that cells underwent chromosomal rearrangements and plasmid loss. Furthermore, proteomics analysis revealed the key differences in metabolic pathways responsible for the existence of cells. After prolonged starvation of cells the numerous proteins engaged in glucose and amino-acid catabolizing pathways, glycerolipid metabolizing pathways, and stress-response mechanisms were all differentially expressed. Metabolomics analysis indicated that the studied cells produce less lactic acid in comparison to parental cells. The understanding of experimental evolution, such as the long-term adaptation, may facilitate new strategies for development of cell lines with advantageous traits.
Type of meeting: Predavanje
Type of presentation in a journal: Abstract
Type of peer-review: Domestic peer-review
Project / theme: 058-0583444-3466, 098-0000000-3454
Original language: ENG
Category: Ostalo
Research fields:
Contrib. to CROSBI by: Ana Butorac (, 9. Sij. 2014. u 11:14 sati

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