engleski

On-line DACC-HPLC analysis of polycyclic aromatic hydrocarbons in edible oils

In this work an HPLC method for determination of polycyclic aromatic hydrocarbons (PAH) in edible oils on a DACC (donor-acceptor complex chromatography) column coupled with an on-line HPLC system with fluorescent detection. Method was used to determine the content of individual PAHs in the refined sunflower oil, virgin olive oil, cold pressed pumpkin seed oil, dark sesame oil and pumpkin seed oil produced with roasting obtained from domestic market. Calibration and validation were conducted for 13 individual PAHs among which are all 8 PAHs that are listed as priorities for determination in food and environment by both European Commission and US Environmental Protection Agency (EPA). Correlation coefficients of calibration curves for all standards were above 0.999 in selected range and all validation parameters (limit of detection, limit of quantification, accuracy and precision) were within limits set by European regulation. Content of PAHs in cold pressed pumpkin seed oil, roasted pumpkin seed oil and especially dark sesame oil was several-fold higher than the recommendations set by different European oil research and production organizations even though for both pumpkin seed oils it was lower than limits set by official European legislation. High content of cancerogenous heavy fraction PAHs were found in dark sesame oil that advises to limitation of its consumption in human diet.

polycyclic aromatic hydrocarbons; DACC-HPLC analysis; method development; method validation; edible oils

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