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Bibliographic record number: 786828

Journal

Authors: Križanović, Stela; Butorac, Ana; Mrvčić, Jasna; Krpan, Maja; Cindrić, Mario; Bačun-Družina, Višnja; Stanzer, Damir
Title: Characterization of a S-adenosyl-L-methionine (SAM)-accumulating strain of Scheffersomyces stipitis
Source: International microbiology (1139-6709) 18 (2015), 3; 117-125
Paper type: article
Keywords: Scheffersomyces stipitis • S-adenosyl-L-methionine (SAM); SAM accumulating yeast; C-24 sterol methyltrans-ferase (Erg6p)
Abstract:
S-adenosyl-L-methionine (SAM) is an important molecule in the cellular metabolism of mammals. In this study, we examined several of the physiological characteristics of a SAM-accumulating strain of the yeast Scheffersomyces stipitis (M12), including SAM production, ergosterol content, and ethanol tolerance. S. stipitis M12 accumulated up to 52.48 mg SAM/g dry cell weight. Proteome analyses showed that the disruption of C-24 methylation in ergosterol biosynthesis, a step mediated by C-24 sterol methyltransferase (Erg6p), and results in greater SAM accumulation by S. stipitis M12 compared to the wild-type strain. A comparative proteome-wide analysis identified 25 proteins that were differentially expressed by S. stipitis M12. These proteins are involved in ribosome biogenesis, translation, the stress response, ubiquitin-dependent catabolic processes, the cell cycle, ethanol tolerance, posttranslational modification, peroxisomal membrane stability, epigenetic regulation, the actin cytoskeleton and cell morphology, iron and copper homeostasis, cell signalling, and energy metabolism.
Project / theme: 058-0583444-3483, 058-0580477-0374, 058-0583444-3466
Original language: ENG
Citation databases: Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Category: Znanstveni
Research fields:
Biotechnology,Food processing technology
URL: http://revistes.iec.cat/index.php/IM/article/view/139217
URL cjelovitog rada:
Google Scholar: Characterization of a S-adenosyl-L-methionine (SAM)-accumulating strain of Scheffersomyces stipitis
Contrib. to CROSBI by: Višnja Bačun-Družina (visnjabd@pbf.hr), 20. Stu. 2015. u 12:35 sati



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