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Bibliographic record number: 891097

Journal

Authors: Jakopović, Željko; Mrvčić, Jasna; Pleadin, Jelka; Barišić, Lidija; Frece, Jadranka; Čanak, Iva; Markov, Ksenija
Title: FT-IR spectroscopy as a method to detected cell surface components of Gluconobacter oxydans involved in the bacteria-OTA interaction
Source: Journal of Biotechnology, volume 256, Supplement
Meeting: EUROPEAN BIOTECHNOLOGY CONGRESS
Location and date: Dubrovnik, Hrvatska, 25-27.5.2017.
Keywords: Gluconobacter oxydans, OTA, cell wall components, FT-IR
Abstract:
Fourier Transform Infrared Spectroscopy (FT-IR) is an analytical technique, which provides unique spectrum for each bacterial species due to the stretching and bending vibrations of functional groups present in cell proteins, nucleic acids, lipids, sugars, and lipopolysaccharides. The potential of Gluconobacter oxydans to bind ochratoxin A (OTA) on surface and changes occurring on the cell surface due to mycotoxin exposure were investigated using the FT-IR. OTA was determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) and the results suggest that G. oxydans is able to bind or degrade OTA by 81%. The results revealed capacity of OTA exposed cells to bind a high amount of the toxins is mirrored in the alteration of the original IR patterns, the latter alteration arising due the presence of numerous functional groups within the toxin structure. Major changes seen in the 1, 800-1, 600 cm-1, 1, 600-1, 450 cm-1 and 1, 300-1, 000 cm-1 regions, mainly correspond to carbonyl, aromatic and ether groups, respectively. These groups are abundant in OTA and increase the existing, or provide new spectrum bands. Additionally, very strong signals in the organic halogen compounds region at 870 cm–1, probably corresponds to the C-C1 vibration taking place in OTA.
Type of meeting: Poster
Type of presentation in a journal: Abstract
Type of peer-review: International peer-review
Original language: ENG
Category: Ostalo
Research fields:
Biotechnology,Food processing technology
Contrib. to CROSBI by: Željko Jakopović (zjakopovic@pbf.hr), 11. Ruj. 2017. u 08:01 sati



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