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Pregled bibliografske jedinice broj: 192668


Autori: MacDonald, Daniel; Marković, Berislav; Allen, Mike; Somasundaran, Ponisseril; Boskey, Anell
Naslov: Surface analysis of human plasma fibronectin adsorbed to commercially pure titanium materials
Izvornik: Journal of biomedical materials research (0021-9304) 41 (1998); 120-130
Vrsta rada: članak
Ključne riječi: implant surface; titanium; fibronectin; atomic force microscopy; contact angle
Protein binding on metallic implant surfaces, such as titanium, is governed by the physico-chemical nature of the metallic surface. Human plasma fibronectin (HPF) is an important matrix glycoprotein that mediates cell and protein attachment to each other or to the extracellular matrix present during wound healing. The objective of this study was to investigate the adsorption of HPF onto polished commercially pure titanium (cpTi) by using atomic force microscopy (AFM) and electron spectroscopy for chemical analysis (ESCA) and to measure the resultant surface contact angle before and after HPF binding. Two types of cpTi disks, one highly polished in our laboratory (HSS) and one commercially prepared (31), were reacted with HPF solutions of varying concentrations (1 g/mL - 10 ng/mL). ESCA survey spectra of samples coated with 1 g/mL of fibronectin showed an increase in organic nitrogen and carbon compared with uncoated controls. Contact angle measurements of HSS and 3I cpTi disks showed no significant difference in average contact angle (36.3° ± ; ; 3.5 and 39.1° ± ; ; 3.1) despite differences in local root mean square (RMS) surface roughness (4.45 ± ; ; 0.46 nm and 22.37 ± ; ; 4.17 nm) as measured by AFM. Images obtained by AFM showed that 3I specimens were more irregular, with large parallel polishing grooves. Adsorbed HPF appeared in a globular form with an average length of 16.5 ± ; ; 1.0 nm, a height of 2.5 ± ; ; 0.5 nm, and a width of 9.6 ± ; ; 1.2 nm. Fibronectin coating on both HSS and 3I cpTi specimens resulted in a significant increase in hydrophobicity compared to uncoated specimens. These results indicate the significance of HPF on cpTi and may explain how cpTi implants function in situ.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija,Temeljne medicinske znanosti,Dentalna medicina
URL cjelovitog teksta:
Google Scholar: Surface analysis of human plasma fibronectin adsorbed to commercially pure titanium materials

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