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Bibliographic record number: 366412


Authors: Belščak, Ana; Komes, Draženka; Piljac Žegarac, Jasenka
Title: Antioxidant potential of Mediterranean herbs and herb-based honeys
Source: Book of abstracts / 15th Young Investigators´ Seminar on Analytical Chemistry / Prosen, Helena (ed). - Ljubljana : Faculty of Chemistry and Chemical Technology, University of Ljubljana , 2008. 46-49 (ISBN: 978-961-6286-96-1).
Meeting: 15th Young Investigators´ Seminar on Analytical Chemistry
Location and date: Ljubljana, Slovenija, 02-05. 07. 2008.
Keywords: antioxidants; honey; medicinal herbs; polyphenols
In the past few years, an increasingly growing interest in functional foods has triggered a large number of studies on phenolic antioxidants in plant-based products. Polyphenolic phytochemicals in native Mediterranean herbs, such as laurel (Laurus nobilis), basil (Basilicum ocimum), wild thyme (Thymus vulgaris L.), sage (Salvia officinalis L.) and lavender (Lavandula angustifolia Mill.), act as powerful antioxidants, and their application in functional food development has a significant potential. Honey exhibits many therapeutical properties that have been exploited in traditional medicine, and is easily accessible to most consumers. Until recently, the focus of the scientific community has been directed more to the other honey components such as amino acids, proteins, trace elements, sugars and pollen [1-3], rather than antioxidants. Many positive health effects of honey may be explained by the synergystic action of phenolic compounds. The purpose of our study was to determine the polyphenolic content and antioxidant capacity of commonly used herbal spices, herb-based honeys and special honey preparations (with added fruit or vegetable components). The Folin-Ciocalteu assay was used to determine the total phenol (TP) content, ferric reducing antioxidant power (FRAP) assay for reducing capacity and 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and 2, 2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays for radical scavenging capacity. High performance liquid chromatography (HPLC) was applied for identification of individual polyphenolic compounds in herbal spices. Significant linear correlation was obtained between the results of different methods, indicating that phenolics are mostly responsible for the reducing and antiradical capacity of tested herbs and honeys. The most abundant polyphenolic compound in herbal spices was rosmarinic acid (RA), extracted from wild thyme (20.45 mg/g dw). Among the tested herbal spices, the highest content of phenolic compounds was determined in wild thyme (48.5 mg GAE/g dw), which also exhibited the highest reducing power (4290  mol/L Fe(II)) and antiradical capacity (2.20 and 2.78 mmol/L Trolox in DPPH and ABTS assays, respectively), while lavender extract was characterized by the lowest content of phenolic compounds (8.7 mg GAE/g dw), as well as the lowest reducing power (960  mol/L Fe(II)) and antiradical capacity (0.40 and 0.64 mmol/L Trolox in DPPH and ABTS assays, respectively). In comparing the results for each particular method across all tested honeys, honey with cherry juice exhibited the highest FRAP value (459.21  mol/L Fe(II)) as well as DPPH and ABTS radical scavenging capacities (43.04 and 65.24 mg ascorbic acid equivalents AAE/100 g, respectively), while amorpha honey exhibited very low FRAP value (23.02  mol/L Fe(II)) and DPPH radical scavenging capacity (6.68 mg AAE/100 g), as well as the lowest ABTS radical scavenging capacity (6.83 mg AAE/100 g). The results obtained in this study verify the significant in vitro antioxidant properties of herbal spices, due to their high content of polyphenolic compounds, in particular rosmarinic acid. These findings also point to increased antioxidant capacity of special honey preparations containing various fruit and herbal components. The displayed results indicate that herbal spices, beside acting as astringents and flavoring agents, may also enhance the already confirmed beneficial effects of honey.
Type of meeting: Predavanje
Type of presentation in a journal: Abstract
Type of peer-review: International peer-review
Project / theme: 098-0982913-2829, 058-0000000-3470
Original language: ENG
Category: Znanstveni
Research fields:
Biotechnology,Food processing technology
Contrib. to CROSBI by: (, 25. Ruj. 2008. u 08:16 sati

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