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Bibliographic record number: 700267


Authors: Komes, Draženka; Belščak-Cvitanović, Ana; Chassaing, Anaïs; Bušić, Arijana; Vojvodić, Aleksandra
Title: Comparative study of carbohydrates and polyphenols in carob (Ceratonia siliqua L.) and cocoa husk (Theobroma cocoa L.) affected by different extraction techniques and solvents
Source: Book of abstracts: 7th Central European Congress on Food / Kakurinov, Vladimir (ed). - Skopje : Consulting and Training Centar KEY , 2014. 24 (ISBN: 978-608-4565-05-5).
Meeting: 7th Central European Congress on Food
Location and date: Ohrid, Makedonija, 21-24.05.2014.
Keywords: Antioxidant capacity; Carob; Cocoa husks; Dietary fibres; Polyphenols; Sugars
Biologically active compounds although being native constituents of food, are not consumed in a sufficient extent as a consequence of irregular and unbalanced diet. Therefore increasing interest arises in exploiting naturally derived plant products as sources of bioactive ingredients, in order to increase their intake and positive health effects. Among plant materials, carob (Ceratonia siliqua L.) and cocoa husk (Theobroma cocoa L.) have in common a high content of dietary fibres and polysaccharides, but a lack of information exists regarding their antioxidant capacity and polyphenolic profile. In this study the bioactive composition and antioxidant properties, as well as the content of dietary fibers and sugars in carob and cocoa husk, affected by different extraction techniques (hydrolysis, ultrasonic, conventional) and solvents (aqueous mixtures of acetone and ethanol) was evaluated using spectrophotometric and high performance liquid chromatography (HPLC) methods. Cocoa husk exhibited higher total dietary fibre content, but lower content of fructose and glucose, in comparison to carob, which was also characterized with a significant content of sucrose. Both acid and alkali hydrolysis of raw materials provided the highest content of polyphenolic compounds and antioxidant capacity, which could be attributed to interferences in the measurement procedure and non-selectivity of employed reagents. By comparing non-hydrolysed samples, the highest total phenol content (TPC) was determined in extracts prepared using 80% acetone (carob-15.2 mg gallic acid equivalents/g DM, cocoa husk-4.8 mg GAE/g DM). The ranking of samples according to their total flavonoid content and antioxidant capacity corresponds to their TPC order (80% acetone > 50% ethanol > 70% ethanol). HPLC analysis revealed the prevalence of methylxanthines in cocoa husk and gallic acid and its derivatives in carob. The evaluated plant materials represent rich sources of carbohydrates, as well as polyphenolic antioxidants, while the content of these compounds is dependent on the employed extraction technique and solvent.
Type of meeting: Poster
Type of presentation in a journal: Abstract
Type of peer-review: International peer-review
Project / theme: 058-0000000-3470
Original language: ENG
Category: Znanstveni
Research fields:
Food processing technology
Contrib. to CROSBI by: Arijana Bušić (, 5. Lip. 2014. u 14:31 sati

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