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Flavonoids in Croatian Chestnut (Castanea sativa) honey

All honey types can be generally described as supersaturated sugar solutions, but at the same time they differ in components, such as flavonoids, which are present in small amounts but are responsible for many of their specific properties. The aim of this study was to determine the content of flavonoids in Croatian unifloral chestnut (Castanea sativa) honey. For that purpose 9 chestnut honey samples, for which characterisation was achieved by the combination of physicochemical properties and pollen analysis, have been analysed. Flavonoid fraction was extracted from honey and then analysed using reversed-phase high performance liquid chromatography (RP-HPLC) method. Flavonoids myricetin, quercetin, luteolin, kaempferol, apigenin, isorhamnetin, chrysin and galangin were identified and quantified in each sample. Total amount of identified flavonoids varied from 149 μg/100 g of honey to 313 μg/100 g of honey, with the average of 231 μg/100 g of honey. All analysed samples showed common flavonoid profile.

Croatian unifloral chestnut honey; flavonoids; RP-HPLC analysis

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