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Bibliographic record number: 536545

Journal

Authors: Grubišić, Leon; Šegvić-Bubić, Tanja; Lepen Pleić, Ivana; Mišlov, Krstina; Tičina, Vjekoslav; Katavić, Ivan; Mladineo, Ivona
Title: Morphological and genetic identification of spontaneously spawned larvae of captive Bluefin Tuna Thunnus thynnus in the Adriatic Sea
Source: Fisheries (0363-2415) 38 (2013), 9; 410-417
Paper type: article
Keywords: Bluefin Tuna; Thunnus thynnus; spawning; Adriatic Sea
Abstract:
The spawning behavior of captive Bluefin Tuna (Thunnus thynnus) was observed in cages at an Adriatic facility in summer 2011. Approximately 20, 000 eggs were collected from cages and transferred to a land-based nursery, with an estimated fertilization success rate of 80%. Eggs were spherical with a diameter of 1, 035.06 ± 20.16 μm and were pelagic with a single oil globule (227.63 ± 8.07 μm). Newly hatched larvae (3.08 ± 0.14 mm total length) followed scombrid larval development: melanophores were scattered over the body, head, notochord, and yolk, except finfold ; eye pigmentation was observed 1.5 days posthatch, pectoral fins started to appear, and two-thirds of the yolk sac were absorbed ; the mouth developed at 2.5 days posthatch, and larvae began feeding upon enriched rotifers. After mortalities occurred in the nursery (5 days posthatch), DNA was extracted from a sample of larvae. An 890 base pair fragment of the mitochondrial partial control region was amplified and sequenced to genetically confirm fish species identity. Based on multiple sequence alignment, larval sequences showed no ambiguities to the T. thynnus mitochondrial control region, thereby confirming spontaneous spawning in captivity. Although such an event has previously been reported in the Mediterranean, this is the first report supported by both morphological and genetic data.
Project / theme: 001-0000000-3633, 001-0010501-0560
Original language: ENG
Citation databases: Web of Science: Science Citation
Current Contents Citation Index
Scopus
Web of Science: Science Citation Index Expanded
Category: Znanstveni
Research fields:
Veterinary medicine,Biotechnology
URL: http://www.tandfonline.com/doi/full/10.1080/03632415.2013.826201#.U1pIWqJqOoY
Broj citata:
Altmetric:
DOI: 10.1080/03632415.2013.826201
URL cjelovitog rada:
Google Scholar: Morphological and genetic identification of spontaneously spawned larvae of captive Bluefin Tuna Thunnus thynnus in the Adriatic Sea
Contrib. to CROSBI by: mladineo@izor.hr (mladineo@izor.hr), 10. Stu. 2011. u 14:28 sati



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