The Use of Mass Spectrometry in Characterization of Bone Morphogenetic Proteins from Biological Samples (CROSBI ID 50378)
Prilog u knjizi | stručni rad
Podaci o odgovornosti
Razdorov, Genadij ; Vukicevic, Slobodan
engleski
The Use of Mass Spectrometry in Characterization of Bone Morphogenetic Proteins from Biological Samples
Growth factors MS based characterization from biofluids is almost exclusively done by CID on plethora of different tandem instruments. Very low levels of growth factors in circulation is limiting their MS based identification due to huge dynamic range of plasma/serum proteins. Different biochemical purification strategies are used to enrich individual growth factors enough to be able to analyse it by MS. Most of them use heparin affinity chromatography as one purification step. We have successfully identified number of growth factors from biofluids using heparin based enrichment, followed by the SDS-PAGE protein separation and peptide analysis on nano LC coupled to LTQ-Orbitrap.
Mass Spectrometry, Bone Morphogenetic Proteins, BMPs
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Podaci o prilogu
259-284.
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