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Pregled bibliografske jedinice broj: 101574

Časopis

Autori: Gabriel, Matthew N.; Calloway, Cassandra D.; Reynolds, Rebecca L.; Anđelinović, Šimun; Primorac, Dragan
Naslov: Population variation of human mitochondrial DNA hypervariable regions I and II in 105 Croatian individuals demonstrated by immobilized sequence-specific oligonucleotide probe analysis
Izvornik: Croatian medical journal (0353-9504) 42 (2001), 3; 328-335
Vrsta rada: članak
Ključne riječi: DNA; mitochondrial; forensic medicine; genetic markers; haplotypes; oligonucleotide probes; polymerase chain reaction; short tandem repeat
Sažetak:
Aim. To detect sequence variation in 105 Croatian individuals by the use of duplex polymerase chain reaction amplification of full-length hypervariable region I and II (HVI/HVII) products and subsequent hybridization to a linear array of 27 immobilized sequence-specific oligonucleotide (SSO) probes, which targets six regions within HVI and HVII, and two additional sites, 189 and 16093. Methods. Chelex-extracted bloodstains were used for amplification of HV regions. In all cases, a single robust amplification was sufficient for immobilized SSO probe typing and subsequent direct sequence analysis for both HVI and HVII. This method, suitable for a range of forensic samples (including shaft portions of single hairs), was also applied to the analysis of 18 skeletal elements recovered from a mass grave. Using a panel of immobilized SSO probes, we have developed a rapid screening approach to mitochondrial DNA (mtDNA) haplotyping before direct sequence analysis. Results. We established a reference sequence database of mtDNA haplotypes for 105 randomly selected Croatian individuals. Fifty different mitotypes were observed (33 unique). The most frequent mitotypes occurred 18 times or similar to 17.1% [111111 189 (A) 16093 (T)] and 11 times or similar to 10.5% [131111 189 (A) 16093 (T)] ; all other mitotypes occurred 5% or less. The corresponding genetic diversity value for this database was similar to0.952. The usefulness of establishing an mtDNA reference database with immobilized SSO probe testing has been demonstrated by determining the strength of a match comparison obtained for one skeletal element and a corresponding maternal reference from 18 specimens recovered from a mass grave. Conclusion. The sequence variation detected by the panel of immobilized SSO probes is sufficiently diverse to be used for identification of human skeletal remains from mass graves. The immobilized SSO typing strip targets polymorphic regions within HVI and HVII and is a useful identification tool for mass grave and mass disaster analysis, as well as for criminal casework testing.
Projekt / tema: 141009
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
Puni text rada: 101574.2001_CMJ_Gabriel_Calloway_br_3_str_328_Population_variation.pdf (tekst priložen 28. Ožu. 2007. u 14:26 sati)
URL cjelovitog teksta:
Google Scholar: Population variation of human mitochondrial DNA hypervariable regions I and II in 105 Croatian individuals demonstrated by immobilized sequence-specific oligonucleotide probe analysis



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