engleski

Aplication of RAPD analysis for identification of Lactococcus lactis subsp. cremoris strains isolated from artisanal cultures

Randomly amplified poplymorphic DNA (RAPD) was used for identification of Lactococcus lactis subsp. cremoris strains isolated 40 years ago from various dairy homemade products. Total genomic DNAs from six randomly chosen isolates and the reference strain Lactococcus lactis subsp. cremoris NIZO B64 were amplified using four different 10-mer primers. Although most RAPD fragments were commom to all six isolates, a sufficient number of polymorphic fragments were also detectedthat allowed clear distincion of the isolates and the reference strain. The results indicate that RAPD analysis could be a useful and efficient method to distiguish Lactococcus lactis subsp. cremoris at the strain level and to dettect genetic diersity.

Artisanal cultures; Lactococcus lactis subsp. cremoris; strain identification; RAPD analysis; PCR

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