Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Comparison of protective effects of catechin applied in vitro or in vivo on ischemia-reperfusion injury in the isolated rat hearts (CROSBI ID 103409)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Modun, Darko ; Musić, Ivana ; Katalinić, Višnja ; Salamunić, Ilza ; Boban, Mladen Comparison of protective effects of catechin applied in vitro or in vivo on ischemia-reperfusion injury in the isolated rat hearts // Croatian medical journal, 44 (2003), 6; 690-696-x

Podaci o odgovornosti

Modun, Darko ; Musić, Ivana ; Katalinić, Višnja ; Salamunić, Ilza ; Boban, Mladen

engleski

Comparison of protective effects of catechin applied in vitro or in vivo on ischemia-reperfusion injury in the isolated rat hearts

Aim. To determine and compare overall cardiac effects of flavonoid catechin, applied in vitro or in vivo, on ischemia-reperfusion injury in isolated rat hearts. Methods. After perfusing hearts at 70 mm Hg, coronary perfusion was interrupted for 30 minutes and then reestablished. There were four experimental groups (n=10 each): (I) 100 &micro ; ; ; M/L of catechin added to the perfusate 10 minutes before, during and 10 minutes after ischemia ; (II) in vivo pretreatment with catechin (250 mg/kg b.w.) applied intragastrically for 10 days or (III) as a single dose 1 hour before sacrifice ; and (IV) control. An additional group of 10 hearts served as untreated, nonischemic time control. The variables included heart rate, atrioventricular conduction time, cardiac rhythm, isovolumetric left ventricular pressure, coronary flow and responsiveness, oxygen consumption, relative cardiac efficiency, lactate dehydrogenase release, and myocardial lipid peroxidation. Results. Catechin added to the perfusate increased the coronary flow and ratio of oxygen delivery to myocardial oxygen consumption, before ischemia and during reperfusion. Following 30 minutes of reperfusion, isovolumetric left ventricular developed pressure recovered to 42&plusmn ; ; ; ; ; ; ; 3%, 63&plusmn ; ; ; ; ; ; ; 3%, 71&plusmn ; ; ; ; ; ; ; 2%, and 55&plusmn ; ; ; ; ; ; ; 3% of the initial control values for control, catechin 1 hour, catechin for 10 days and catechin in vitro group, respectively. Cardiac efficiency and coronary responsiveness were also best preserved in the group receiving catechin for 10 days. Conclusion. Application of catechin both in vitro, and short and long term in vivo, resulted in cardioprotection against ischemia-reperfusion injury, but long term pretreatment provided more favorable effects. Directly applied, catechin acted as a vasodilator.

catechin; ischemia-reperfusion; heart

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

44 (6)

2003.

690-696-x

objavljeno

0353-9504

Povezanost rada

Kemija, Temeljne medicinske znanosti, Prehrambena tehnologija

Indeksiranost