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Pregled bibliografske jedinice broj: 139141

Zbornik radova

Autori: Dekaris, Iva; Gabric, Nikica; Markotic, Alemka; Lazic, Ratimir; Cima, Ivan; Bosnar, Damir;
Naslov: TNF-a and IL-1a production by diseased human corneas
( TNF-a and IL-1a production by diseased human corneas )
Izvornik: Abstract book of Arvo / Anual Meeting
Skup: Arvo, Anual Meeting
Mjesto i datum: Fort Lauderdale, Florida, SAD, 04-09.05.2003
Ključne riječi: cytokine levels; TNF alpha; IL-alpha; proinflammatory citokines
( cytokine levels; TNF alpha; IL-alpha; proinflammatory citokines )
Sažetak:
Purpose. Purpose of this study was: a) to determine pro-inflammatory cytokine secretion from cultured human corneas with different pathology, and b) to establish whether cytokine profile at the time of surgery influenced corneal graft outcome. Methods. Proinflammatory (TNF-a and IL-1a ) cytokine secretion was measured after 24 hour cultivation of diseased human corneas. 47 corneas were collected from corneal graft receipients suffering from different corneal diseases. Non-inflammatory corneal diseases included: keratoconus (n=8), keratoglobus (n=2), bullous keratopathy (n=11), and stromal dystrophy (n=2). Inflammatory diseases included vascularised corneal scars (n=14), graft rejection (n=6) and corneal ulcer (n=4). Corneas were cultivated at 37oC for 24 hours and frozen at -21oC till cytokine detection. TNF-a and IL-1a levels were measured by immunoassay (R&D Systems, USA). Corneas unsuitable for transplantation due to low endothelial cell count (obtained from the eye bank) were used as controls. Statistical analysis was made by Student t-test. Results. The concentration of IL-1a secreted from corneas with non-inflammatory diseases was 5.56 ± ; 4.03 pg/mm3. In inflammatory corneal diseases, the concentration of IL-1a was significantly higher (11.4 ± ; 5.58 pg/mm3, p<0.05). Low level of TNF-a was detected only in case of vascularised leucoma, bullous keratopathy and graft rejection (1.85 ± ; 1.82 pg/mm3, 0.38 ± ; 0.7 pg/mm3 and 0.56 ± ; 0.81 pg/mm3 respectively). Rejection rate in inflammatory group was higher as compared to non-inflammatory one. Conclusion. Increased production of IL-1a by corneal recipients with inflammatory diseases suggests its role in corneal graft rejection in humans, similar as it was already found in animal model.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 0129010
Izvorni jezik: eng
Kategorija: Znanstveni
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