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Pregled bibliografske jedinice broj: 151043

Zbornik radova

Autori: Cvetko, Lidija; Markotić, Alemka; Plyusnina, Angelina; Turk, Nenad; Margaletić, Josip; Milas, Zoran; Miletić-Medved, Marica; Avšič-Županc, Tatjana; Plyusnin, Alexander
Naslov: Genetic analysis of Puumala virus in newly discovered endemic region during great 2002 HFRS outbreak in Croatia
( Genetic analysis of Puumala virus in newly discovered endemic region during great 2002 HFRS outbreak in Croatia )
Izvornik: Conference Program & Abstracts
Skup: The 6th International Conference on Hemorrhagic Fever with Renal Syndrome (HFRS), Hantavirus Pulmonary Syndrome (HPS) and Hantaviruses
Mjesto i datum: Seoul, Južna Koreja, 23-25.06.2004.
Ključne riječi: HVBS; hantavirusi; Puumala; genetska analiza
( HFRS; hantaviruses; Puumala; genetic analysis )
Sažetak:
Puumala virus (PUUV), causative agent of the mild type of hemorrhagic fever with renal syndrome (HFRS) belongs to the Hantavirus genus in the Bunyaviridae family. PUUV was shown to be dominant pathogen during the great HFRS outbreak in Croatia in year 2002. We focused our research on two newly discovered endemic areas (Okucani and Nova Gradiska) with the high number of reported HFRS cases and a significant increase of rodents population. Genetic analysis of wild-type (wt) Puumala virus strains from Croatia was performed. Strains were gained from its natural host reservoirs Clethrionomys glareolus originating from PUU-associated HFRS areas. Fifty seven bank voles were screened for the presence of PUU N antigen and 15 (26 %) were found to be positive. Total RNA isolated from rodent lung tissues was reverse transcribed followed by PCR amplification with medium (M) segment gene-specific primers. Partial sequences approximately 450 bp long of the wt-PUU M genome segment were recovered from the five C. glareolus. Phylogenetic analysis of the Croatian wt-PUU strains from the two localities revealed close relatedness of all five strains but without visible geographical clustering of genetic variants. On phylogenetic tree Croatian PUU strains clustered together with the strains from Austria. This confirms the idea that Croatian PUU falls into south-european genetic lineage, as was expected. Along with HFRS, another zoonosis - leptospirosis is very common at the same regions. Both etiologic agents are rodent-borne and are maintained in persistently infected small rodents. They share same rodent species as host reservoirs. It is known that one rodent species can carry more different pathogens. We confirmed existence of PUUV (by PCR) and a strain of Leptospira spp (isolation) in the same C. glareolus.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 0021005, 0021001
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti



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