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Pregled bibliografske jedinice broj: 151044

Zbornik radova

Autori: Cebalo, Karin; Cvetko, Lidija; Markotić, Alemka; Gagro, Alenka; Lončar, Ljubica; Sladoljev, Srećko; Rabatić, Sabina
Naslov: Propagation of Sendai virus on MRC-5 cells
( Propagation of Sendai virus on MRC-5 cells )
Izvornik: Animal Cell Technology meets Genomics / Godia, Francesc (ur.). - Granada :
Skup: 18th ESACT MEETING
Mjesto i datum: Granada, 11-14.05.2003.
Ključne riječi: Sendai virus; MRC-5
( Sendai virus; MRC-5 )
Sažetak:
Sendai virus (SeV) belongs to the family Paramyxoviridae and structurally is an enveloped, single stranded negative sense RNA virus. It is highly cytopathic virus in different cell cultures, where it can induce apoptosis. Previous studies showed that human foetal lung fibroblasts (MRC-5) are suitable host cells for numerous viruses. We wanted to investigate whether SeV (usually propagated in the allantoic cavity of the chicken embryo) can grow on MRC-5 cells also. The virus titre was determined by the hemaglutination test. The first passage of SeV on MRC-5 cells gave the highest titre after two days and started to decline after day four, while in the second SeV passage, no virus titre was found. SeV induced cytopathic effect in MRC-5 cells. Previously it was found that SeV induced FAS (CD95)-independent apoptosis pathway in CV-1 cells. CD95 is a surface molecule that mediates apoptotic death on infected cells. We measured the expression of CD95 by flow cytometry and our preliminary results showed downregulation of CD95 expression on MRC-5 cells infected with SeV (propagated on chicken embryo). To investigate the possible role of cytokines/chemokines in the interaction of SeV with MRC-5 cells, we determined their levels in supernatants of infected cells by ELISA. Tumour necrosis factor alpha (TNF-alpha), interferon alpha (IFN-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-6, IL-10, IL-12p70, IL-8, and macrophage chemoattractant protein-1 (MCP-1) were measured. In the first passage, SeV induced the production of MCP-1, GM-CSF and IL-6, while in the second passage only low IL-6 level was detected. Further studies are necessary to shed more light on the mechanisms involved in the interaction between SeV and MRC-5 cells.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Nije objavljen
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 0021001, 0021005
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti



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