crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 151083

Zbornik radova

Autori: Matijević, Ivana; Globočnik, Tina; Cebalo, Ljiljana; Cvetko, Lidija; Cebalo, Karin; Jelačić, Jasenka; Baletić, Jagoda; Gotovac, Katja; Kosor, Ela; Sabioncello, Ante; Vugrinec Kunštek, Ksenija; Rabatić, Sabina; Kuzman, Ilija; Puljiz, Ivan; Rode, Oktavja; Turk, Nenad; Milas, Zoran; Margaletić, Josip; Miletić-Medved, Marica; Avšič-Županc, Tatjana; Markotić, Alemka
Naslov: Specificity and sensitivity of diagnostic rapid tests for the acute infections with Hantaviruses
( Specificity and sensitivity of diagnostic rapid tests for the acute infections with Hantaviruses )
Izvornik: Conference Program & Abstracts of The 6th International Conference on Hemorrhagic Fever with Renal Syndrome (HFRS), Hantavirus Pulmonary Syndrome (HPS) and Hantaviruses / Soon-Bong Hwang (ur.). - Seoul :
Skup: International Conference on Hemorrhagic Fever with Renal Syndrome (HFRS), Hantavirus Pulmonary Syndrome (HPS) and Hantaviruses (6 ; 2004)
Mjesto i datum: Seoul, Južna Koreja, 23.-25.06.2004.
Ključne riječi: Immunochromatographic test; Puumala; Dobrava; Hemorrhagic fever with renal syndrome (HFRS)
( Immunochromatographic test; Puumala; Dobrava; Hemorrhagic fever with renal syndrome (HFRS) )
Sažetak:
Puumala (PUUV) and Dobrava (DOBV) viruses are causative agents of hemorrhagic fever with renal syndrome (HFRS) in Croatia. Whole Croatia except the coast and islands is HFRS endemic region. Although many diagnostic tests are now developed for the serological confirmation of acute HFRS, there is still need for the accurate rapid test. During the greatest HFRS outbreak in Croatia in 2002, we aimed to evaluate rapid immunochromatographic test for the detection of specific IgM antibodies to PUUV and DOBV. For the preliminary, retrospective evaluation, serum samples of 105 acute HFRS patients were tested with five-minutes, POC PUUMALATM and POC DOBRAVATM point-of-care tests (ERILAB, Finland). HFRS diagnosis has been previously confirmed with one of the standardized techniques like IgM and IgG ELISA tests and IFA test for the detection of the specific antibodies to hantaviruses. Additionally, in some cases diagnosis was confirmed by RT-PCR. Further, both tests were tested for the specificity and sensitivity with the sera of acute HFRS patients infected with PUUV or DOBV. To evaluate the specificity of both rapid tests, we tested sera of patients with confirmed other acute zoonozis in Croatia: leptospirosis, tick borne encephalitis (TBE) and borreliosis. To exclude the subjectivity in the interpretation, several evaluators interpreted the tests and differences among them were statistically tested using 2.test. Retrospective evaluation showed that in about 80% of patients acute HFRS diagnosis was confirmed with the rapid immunochromatographic tests only. There were no statistical differences among the evaluators who interpreted the POC PUUMALATM, although the less subjectivity was found when they interpreted tests one hour after preparation. For the evaluation of POC DOBRAVATM test, additional evaluation is necessary to confirm the specificity and sensitivity of the test when tested in Croatian patients. Our results showed that sera of the patients with acute leptospirosis, TBE or borreliosis did not cross-react with POC PUUMALATM and POC DOBRAVATM point-of-care tests. The rapid immunochromatographic test is useful tool for the diagnosis of acute HFRS especially during the outbreak. However, the final diagnosis should be confirmed with additional tests.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Nema recenziju
Projekt / tema: 0108160, 0021005, 0021001, 0068116
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Kliničke medicinske znanosti,Javno zdravstvo i zdravstvena zaštita,Veterinarska medicina



  Verzija za printanje   za tiskati


upomoc
foot_4