Hrvatska znanstvena bibliografija (CROSBI)



Pregled bibliografske jedinice broj: 151254

Časopis

Autori: Marušić, Ana; Markotić, Anita; Kovačić, Nataša; Müthing, Johannes
Naslov: Expression of glycosphingolipids in lymph nodes of mice lacking TNF receptor 1: biochemical and flow cytometry analysis
Izvornik: Carbohydrate research (0008-6215) 339 (2004); 77-86
Vrsta rada: članak
Ključne riječi: glycosphingolipids; gangliosides; lymph nodes; TNF receptor 1; knockout mice; HPTLC immunostaining; flow cytometry
Sažetak:
We investigated the expression of gangliosides and neutral glycosphingolipids (GSLs) in the lymph nodes of mice lacking the gene for the tumour necrosis factor-alpha receptor p55 (TNFR1). The GSL expression in the tissues of mice homozygous (TNFR1-/-) or heterozygous (TNFR1+/-) for the deletion was analysed by flow cytometry and high performance thin layer chromatography (HPTLC) followed by immunostaining with specific antibodies. HPTLC immunostaining revealed that lymph nodes from TNFR1-/- mice had reduced expression of ganglioside GM1b and GalNAc-GM1b, neolacto-series gangliosides, as well as the globo- (Gb3, Gb4 and Gb5) and ganglio-series (Gg3 and Gg4) neutral GSLs. Flow cytometry of freshly isolated lymph node cells showed no significant differences in GSL expression, except for the GalNAc-GM1b ganglioside, which was less abundant on T lymphocytes from TNFR1-/- lymph nodes. In TNFR1-/- mice, GalNAc-GM1b+/CD4+ T cells were two-fold less abundant (3.8% vs. 7.6% in the control mice), whereas GalNAc-GM1b+/CD8+ T cells were four-fold less abundant (5.0% vs. 20.2% in the control mice). This study provides in vivo evidence that TNF signalling via the TNFR1 is important for the activation of GM1b-type ganglioside biosynthetic pathway in CD8 T lymphocytes, suggesting its possible role in the effector T lymphocyte function.
Projekt / tema: 0216013, 0108181
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: www.sciencedirect.com
URL cjelovitog teksta:
Google Scholar: Expression of glycosphingolipids in lymph nodes of mice lacking TNF receptor 1: biochemical and flow cytometry analysis