engleski

Determination of Aucubin and Catalpol in Plantago Species by Mycellar Electrokinetic Chromatography

The genus Plantago L. comprises 265 species and has cosmopolitan distribution. Medicinally, they are astrigents, demulcents, emollients, expectorants, diuretics, antibacterials and antivirals. In recent years, a considerable number of different iridoids have been isolated from the genus Plantago, and these include aucubin, catalpol and compounds biosynthetically related to them. The considerable interest in iridoids has been generated by their relatively wide spectrum of biological activity, as well as by their use as markers in plant taxonomy. Micellar electrokinetic chromatography (MEKC) was used for the separation and determination of two iridoid glycosides, aucubin and catalpol, in several Plantago species growing in Croatia: P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. (subsp. depauperata, subsp. holosteum and subsp. scopulorum), P. lagopus L., P. lanceolata L. and P. maritima L. Hot water extraction (HWE) was applied for the isolation of iridoid substances. Significant differences appeared between the iridoid contents in the examined species. The yield of aucubin and catalpol was up to 0.27% and 1.81% of the dry mass of the drug, respectively. Besides aucubin and catalpol, two iridoid related compounds were determined in plant samples.

Plantago; iridoids; MEKC

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