Hrvatska znanstvena bibliografija (CROSBI)



Pregled bibliografske jedinice broj: 169563

Časopis

Autori: Čačev, Tamara; Jokić, Mladen; Spaventi, Radan; Pavelić, Krešimir; Kapitanović, Sanja
Naslov: Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms
( Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms )
Izvornik: Journal of cancer research and clinical oncology (0171-5216) 132 (2006), 3; 200-204
Vrsta rada: članak
Ključne riječi: real-time PCR; SNP; E-cadherin
( real-time PCR; SNP; E-cadherin )
Sažetak:
Colon cancer is a genetic disease, caused by mutations in different oncogenes and tumor-suppressor genes. The initial escape of a cancer cells from the primary tumor requires changes in cell-cell adhesion switch, which in epithelial tumors, is mediated mainly by members of the cadherin family. The aim of our study was to investigate the LOH at the E-cadherin gene locus in sporadic colon cancer as well as to evaluate the usefulness of real-time PCR SNP analysis as a new technique in this type of studies. 100 cases of human sporadic colon cancer and corresponding normal tissue samples were analyzed using 2 flanking polymorphic markers commonly used in the LOH analysis at the E-cadherin gene locus by conventional VNTR-LOH analysis. Two intragenic E-cadherin SNP markers were analyzed using real-time PCR SNP analysis. 17, 6 % of LOH was detected using flanking markers, however no LOH was detected when the intragenic E-cadherin SNP markers were introduced into our study. Since these markers are intragenic they more accurately represent the status of the E-cadherin gene than the previously used flanking markers. In conclusion, real-time PCR SNP analysis was found to be more accurate, faster, simpler and more high-throughput method than the conventional VNTR-LOH analysis.
Projekt / tema: 0098093, 0098108, 0098109
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://www.springerlink.com/content/pl6t1q80040r2vl3/fulltext.pdf
http://www.springerlink.com/content/pl6t1q80040r2vl3/
Broj citata:
Altmetric:
DOI: 10.1007/s00432-005-0051-y
URL cjelovitog teksta:
Google Scholar: Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms