Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms (CROSBI ID 109243)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Čačev, Tamara ; Jokić, Mladen ; Spaventi, Radan ; Pavelić, Krešimir ; Kapitanović, Sanja
engleski
Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms
Colon cancer is a genetic disease, caused by mutations in different oncogenes and tumor-suppressor genes. The initial escape of a cancer cells from the primary tumor requires changes in cell-cell adhesion switch, which in epithelial tumors, is mediated mainly by members of the cadherin family. The aim of our study was to investigate the LOH at the E-cadherin gene locus in sporadic colon cancer as well as to evaluate the usefulness of real-time PCR SNP analysis as a new technique in this type of studies. 100 cases of human sporadic colon cancer and corresponding normal tissue samples were analyzed using 2 flanking polymorphic markers commonly used in the LOH analysis at the E-cadherin gene locus by conventional VNTR-LOH analysis. Two intragenic E-cadherin SNP markers were analyzed using real-time PCR SNP analysis. 17, 6 % of LOH was detected using flanking markers, however no LOH was detected when the intragenic E-cadherin SNP markers were introduced into our study. Since these markers are intragenic they more accurately represent the status of the E-cadherin gene than the previously used flanking markers. In conclusion, real-time PCR SNP analysis was found to be more accurate, faster, simpler and more high-throughput method than the conventional VNTR-LOH analysis.
real-time PCR; SNP; E-cadherin
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Podaci o izdanju
132 (3)
2006.
200-204
objavljeno
0171-5216
10.1007/s00432-005-0051-y
Povezanost rada
Temeljne medicinske znanosti